|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Bacterially expressed human MCM2|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
|Western Blot (WB)||2-4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
MA5-12333 targets MCM2 in IHC (P) and WB applications and shows reactivity with Human samples.
The MA5-12333 immunogen is bacterially expressed human MCM2.
MCM2 (Mini Chromosome Maintainance protein-2) / BM28 is involved in the control of DNA replication. MCM proteins have DNA dependent ATPase motifs in their central domain which is conserved from yeast to mammals. MCM proteins form a complex which exhibits ATPase and helicase activities. In G0 phase levels of MCM 2 and 5 are lower than that of MCM7 and 3 but increases as the cell enters G1/S phase of the cell cycle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Differential biomarker expression in head and neck cancer correlates with anatomical localization.
MA5-12333 was used in immunohistochemistry to investigate the correlation between the biomarker expression in head and neck cancer and anatomical localization
|Tamás L,Szentkúti G,Eros M,Dános K,Brauswetter D,Szende B,Zsákovics I,Krenács T||Pathology oncology research : POR (17:721)||2011|