|Tested species reactivity||Human, Mouse|
|Host / Isotype||Goat / IgG|
|Immunogen||Synthetic peptide corresponding to a portion of human Minichromosome Maintenance Deficient 3 encoded in part by exons 1 and 2.|
|Purification||Antigen affinity chromatography|
|Storage buffer||tris phosphate/tris citrate, pH 7-8|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:2000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-26581 detects MCM3 from human samples. PA1-26581 is expected to cross react with mouse (98% conserved) due to sequence homology.
PA1-26581 has been successfully used in Western blot procedures.
The PA1-26581 immunogen is a synthetic peptide corresponding to a portion of human Minichromosome Maintenance Deficient 3 encoded in part by exons 1 and 2.
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.