|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Bacterially expressed human MCM5.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1/25 - 1/100|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. A suggested positive control for immunohistochemical applications is tonsil or cervical carcinoma.
Mcm-5 (minichromosome maintenance protein 5) is a protein with an important role in the control of DNA replication. It has been reported that immunocytochemical assessment of Mcm5 expression may be of value in improving accuracy of cervial smear testing for the detection of malignancy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Dormant origins licensed by excess Mcm2-7 are required for human cells to survive replicative stress.
MA1-80807 was used in immunocytochemistry to investigate the role of the dormant origins during the cell cycle progression
|Ge XQ,Jackson DA,Blow JJ||Genes and development (21:3331)||2007|