|Tested species reactivity||Human, Mouse|
|Host / Isotype||Goat / IgG|
|Immunogen||Synthetic peptide corresponding to a portion of human Minichromosome Maintenance Deficient 7 encoded in part by exons 8 and 9.|
|Purification||Antigen affinity chromatography|
|Storage buffer||tris citrate/tris phosphate, pH 7-8|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:2000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-26585 detects MCM7 from human samples. PA1-26585 is expected to cross react with mouse (78% conserved) due to sequence homology.
PA1-26585 has been successfully used in Western blot procedures. Predicted molecular weight: 90 kDa. Positive control: HeLa cell lysate.
The PA1-26585 immunogen is a synthetic peptide corresponding to a portion of human Minichromosome Maintenance Deficient 7 encoded in part by exons 8 and 9.
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.