Immunofluorescent analysis of MCM7 (green) showing staining in the nucleus of C2C12 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a MCM7 monoclonal antibody (Product # MA5-14291) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Actin was stained using Alexa Fluor 554 (red) and nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.
|Tested species reactivity||Dog, Human, Mouse, Rat, Xenopus|
|Published species reactivity||Dog, Rabbit, Mouse, Human|
|Host / Isotype||Mouse / IgG1|
|Clone||47DC141 or DCS-141|
|Immunogen||Recombinant hCDC47 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||0.5-1.0 µg/ml|
|Immunoprecipitation (IP)||2 µg/mg protein lysate|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-14291 targets CDC47 in IF, IHC (P), IP, and WB applications and shows reactivity with Canine, Human, mouse, Rat, and Xenopus laevis samples.
The MA5-14291 immunogen is recombinant hCDC47 protein.
hCDC47 is a human member of the MCM family, which allows DNA to replicate once per cell cycle. In quiescent cells, human MCM7 (hMCM7) mRNA is almost undetectable. Stimulation of cells to enter the cell cycle results in induction of hMCM7 expression. The hCDC47 protein expression and localization is found in nuclei of the proliferative components of normal lymph nodes, bone marrow, epidermis and mucosa. Malignant tumors from several organs contained more nuclear hCDC47-positive cells than their normal counterparts. These results indicate that hCDC47 may play a role in normal and neoplastic cell growth in vivo.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Synonymous codon changes in the oncogenes of the cottontail rabbit papillomavirus lead to increased oncogenicity and immunogenicity of the virus.
MA5-14291 was used in immunohistochemistry to study the increased oncogenicity and immunogenicity of the cottontail rabbit papillomavirus following synonymous mammalian-like changes in codon usage
|Cladel NM,Budgeon LR,Hu J,Balogh KK,Christensen ND||Virology (438:70)||2013|
Encapsulation of cisplatin in long-circulating and pH-sensitive liposomes improves its antitumor effect and reduces acute toxicity.
MA5-14291 was used in immunohistochemistry to study the delivery of cisplatin via pH-sensitive liposomes with a long circulating lifespan to increase antitumor activity whilst reducing acute toxicity
|Leite EA,Souza CM,Carvalho-Júnior AD,Coelho LG,Lana AM,Cassali GD,Oliveira MC||International journal of nanomedicine (7:5259)||2012|
Evaluation of minichromosome maintenance protein 7 as a prognostic marker in canine cutaneous mast cell tumours.
MA5-14291 was used in immunohistochemistry to study the prognostic value of the immunohistochemical expression of MCM7 in canine cutaneous mast cell tumours
|Berlato D,Stewart J,Newton R,Maglennon GA,Monti P,Flindall A,Murphy S||Veterinary and comparative oncology (10:135)||2012|
Persistence of high-grade cervical dysplasia and cervical cancer requires the continuous expression of the human papillomavirus type 16 E7 oncogene.
MA5-14291 was used in immunohistochemistry to study the requirement for continuous expression of the E7 protein of HPV type 16 for the maintenance of high-grade cervical dysplasia and cervical cancer
|Jabbar SF,Abrams L,Glick A,Lambert PF||Cancer research (69:4407)||2009|
Cell proliferation index predicts relapse of brain metastases in non-irradiated patients.
MA5-14291 was used in immunohistochemistry to study the value of proliferative index in predicting brain metastases relapse in non-irradiated patients
|Peev NA,Tonchev AB,Penkowa M,Kalevski SK,Haritonov DG,Chaldakov GN||Acta neurochirurgica (150:1043)||2008|
Critical roles for non-pRb targets of human papillomavirus type 16 E7 in cervical carcinogenesis.
MA5-14291 was used in immunohistochemistry to investigate the effect of non-pRb targets of papillomavirus type 16 E7 on cervical carcinogenesis
|Balsitis S,Dick F,Dyson N,Lambert PF||Cancer research (66:9393)||2006|
Oxidative stress regulated expression of ubiquitin Carboxyl-terminal Hydrolase-L1: role in cell survival.
MA5-14291 was used in western blot to study the role of oxidative stress on ubiquitin carboxy-terminal hydrolase L1 expression and embryonal carcinoma cell survival
|Shen H,Sikorska M,Leblanc J,Walker PR,Liu QY||Apoptosis : an international journal on programmed cell death (11:1049)||2006|
Repression of DNA replication licensing in quiescence is independent of geminin and may define the cell cycle state of progenitor cells.
MA5-14291 was used in western blot to study the role of geminin in the DNA replication licensing during quiescence of progenitor cells
|Kingsbury SR,Loddo M,Fanshawe T,Obermann EC,Prevost AT,Stoeber K,Williams GH||Experimental cell research (309:56)||2005|
Human cytomegalovirus prevents replication licensing by inhibiting MCM loading onto chromatin.
MA5-14291 was used in western blot to study the mechanism by which human cytomegalovirus prevents replication licensing
|Wiebusch L,Uecker R,Hagemeier C||EMBO reports (4:42)||2003|