Invitrogen

MCP-1 Polyclonal Antibody, Biotin, PeproTech®

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MCP-1 Antibody (500-P34BT-1MG) in WB

Western Blot: To detect hMCP-1/MCAF by Western Blot analysis MCP-1 Polyclonal Antibody, Biotin (Product # 500-P34BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hMCP-1/MCAF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

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MCP-1 Antibody in ELISA (ELISA) — Sandwich ELISA: To detect hMCP-1/MCAF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of MCP-1 Polyclonal Antibody, Biotin (Product # 500-P34BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech MCP-1 Polyclonal Antibody (Product # 500-P34-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hMCP-1/MCAF.

Product Details

500-P34BT-1MG

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

ELISA (ELISA)

0.25-1.0 µg/mL

View 12 publications 12 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human MCP-1 (CCL2)

View immunogen

Conjugate

Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Product Specific Information

AA Sequence of recombinant protein: QPDAINAPVT CCYNFTNRKI SVQRLASYRR ITSSKCPKEA VIFKTIVAKE ICADPKQKWV QDSMDHLDKQ TQTPKT.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human MCP-1 (CCL2). Anti-Human MCP-1 (CCL2)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect hMCP-1/MCAF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human MCP-1/MCAF (500-P34) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hMCP-1/MCAF.

Western Blot: To detect hMCP-1/MCAF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hMCP-1/MCAF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P34BT-1MG will be provided as 2 x 500 µg

Target Information

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Tumor Cell-Autonomous Pro-Metastatic Activities of PD-L1 in Human Breast Cancer Are Mediated by PD-L1-S283 and Chemokine Axes.

Cancers

Erlichman N,Baram T,Meshel T,Morein D,Da'adoosh B,Ben-Baruch A

500-P34Bt was used in Enzyme-linked immunosorbent assay to investigate the role of WT-PD-L1 in triple-negative breast cancer.

Fri Feb 18 00:00:00 EST 2022

Tumor-Stroma-Inflammation Networks Promote Pro-metastatic Chemokines and Aggressiveness Characteristics in Triple-Negative Breast Cancer.

Frontiers in immunology

Liubomirski Y,Lerrer S,Meshel T,Rubinstein-Achiasaf L,Morein D,Wiemann S,Körner C,Ben-Baruch A

500-P34Bt was used in Enzyme-linked immunosorbent assay to study the impact of tumor-stroma-inflammation networks on pro-metastatic phenotypes in triple-negative breast cancer (TNBC) to identify a novel tumor-stroma-inflammation networks that may promote TNBC aggressiveness by increasing the pro-malignancy potential of the TME and of the tumor cells themselves, and reveal key roles for CXCL8 in mediating these metastasis-promoting activities.

Mon Aug 10 00:00:00 EDT 2020

Co-Inflammatory Roles of TGFβ1 in the Presence of TNFα Drive a Pro-inflammatory Fate in Mesenchymal Stem Cells.

Frontiers in immunology

Lerrer S,Liubomirski Y,Bott A,Abnaof K,Oren N,Yousaf A,Körner C,Meshel T,Wiemann S,Ben-Baruch A

500-P34Bt was used in Enzyme-linked immunosorbent assay to demonstrate a TNFα + TGFβ1-driven pro-inflammatory fate in MSCs, identify specific molecular mechanisms involved, and propose that TNFα + TGFβ1-stimulated MSCs influence the tumor niche.

Wed Nov 20 00:00:00 EST 2019

M-sec regulates polarized secretion of inflammatory endothelial chemokines and facilitates CCL2-mediated lymphocyte transendothelial migration.

Journal of leukocyte biology

Barzilai S,Blecher-Gonen R,Barnett-Itzhaki Z,Zauberman A,Lebel-Haziv Y,Amit I,Alon R

500-P34Bt was used in Enzyme-linked immunosorbent assay to the first evidence of a novel inflammation-inducible machinery that regulates polarized secretion of endothelial CCL2 and other inflammatory chemokines and cytokines into basolateral endothelial compartments and facilitates the ability of endothelial CCL2 to promote T cell transendothelial migration.

Wed Jun 01 00:00:00 EDT 2016

Regulation of the inflammatory profile of stromal cells in human breast cancer: prominent roles for TNF-α and the NF-κB pathway.

Stem cell research & therapy

Katanov C,Lerrer S,Liubomirski Y,Leider-Trejo L,Meshel T,Bar J,Feniger-Barish R,Kamer I,Soria-Artzi G,Kahani H,Banerjee D,Ben-Baruch A

500-P34Bt was used in Enzyme-linked immunosorbent assay to study the impact of tumour necrosis factor α and interleukin 1β on the inflammatory phenotype of cancer-associated fibroblasts and mesenchymal stem cells.

Fri May 01 00:00:00 EDT 2015

Breast cancer: coordinated regulation of CCL2 secretion by intracellular glycosaminoglycans and chemokine motifs.

Neoplasia (New York, N.Y.)

Lebel-Haziv Y,Meshel T,Soria G,Yeheskel A,Mamon E,Ben-Baruch A

500-P34Bt was used in Enzyme-linked immunosorbent assay to propose that targeting these chemokine regions may lead to reduced secretion of CCL2 by breast cancer cells (and potentially also by other malignant cells).

Mon Sep 01 00:00:00 EDT 2014

The inflammatory cytokine TNFα cooperates with Ras in elevating metastasis and turns WT-Ras to a tumor-promoting entity in MCF-7 cells.

BMC cancer

Leibovich-Rivkin T,Liubomirski Y,Meshel T,Abashidze A,Brisker D,Solomon H,Rotter V,Weil M,Ben-Baruch A

500-P34Bt was used in Enzyme-linked immunosorbent assay to determine the relative contribution of two processes to breast cancer progression: (1) Intrinsic events, such as activation of the Ras pathway and down-regulation of p53; (2) The inflammatory cytokines TNFα and IL-1β, shown in our published studies to be highly expressed in tumors of >80% of breast cancer patients with recurrent disease.

Thu Mar 06 00:00:00 EST 2014

Progression of luminal breast tumors is promoted by ménage à trois between the inflammatory cytokine TNFα and the hormonal and growth-supporting arms of the tumor microenvironment.

Mediators of inflammation

Weitzenfeld P,Meron N,Leibovich-Rivkin T,Meshel T,Ben-Baruch A

500-P34Bt was used in Enzyme-linked immunosorbent assay to indicate that TNF α can turn into a strong prometastatic factor, suggesting a paradigm shift in which clinically approved inhibitors of TNFα would be applied in breast cancer therapy.

Thu Jun 05 00:00:00 EDT 2014

Inflammatory factors of the tumor microenvironment induce plasticity in nontransformed breast epithelial cells: EMT, invasion, and collapse of normally organized breast textures.

Neoplasia (New York, N.Y.)

Leibovich-Rivkin T,Liubomirski Y,Bernstein B,Meshel T,Ben-Baruch A

500-P34Bt was used in Enzyme-linked immunosorbent assay to reveal novel pathways through which the inflammatory microenvironment may contribute to relapsed disease in breast cancer.

Sun Dec 01 00:00:00 EST 2013

Tumor-promoting circuits that regulate a cancer-related chemokine cluster: dominance of inflammatory mediators over oncogenic alterations.

Cancers

Leibovich-Rivkin T,Buganim Y,Solomon H,Meshel T,Rotter V,Ben-Baruch A

500-P34Bt was used in Enzyme-linked immunosorbent assay to use an unbiased model system to propose that inflammatory mediators of the tumor milieu have dominating roles over oncogenic modifications in dictating the expression of a pro-malignancy chemokine readout.

Fri Jan 20 00:00:00 EST 2012

Trans- but not cis-resveratrol impairs angiotensin-II-mediated vascular inflammation through inhibition of NF-κB activation and peroxisome proliferator-activated receptor-gamma upregulation.

Journal of immunology (Baltimore, Md. : 1950)

Rius C,Abu-Taha M,Hermenegildo C,Piqueras L,Cerda-Nicolas JM,Issekutz AC,Estañ L,Cortijo J,Morcillo EJ,Orallo F,Sanz MJ

Wed Sep 15 00:00:00 EDT 2010

STAT4 and STAT6 regulate systemic inflammation and protect against lethal endotoxemia.

The Journal of clinical investigation

Lentsch AB,Kato A,Davis B,Wang W,Chao C,Edwards MJ

Thu Nov 01 00:00:00 EST 2001

Bioinformatics

Protein Aliases: C-C motif chemokine 2; chemokine (C-C motif) ligand 2; H-MCP-1; HC11; MCAF; MCP-1; MGC9434; Monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; Monocyte chemotactic and activating factor; Monocyte chemotactic protein 1; Monocyte secretory protein JE; RP23-350G1.3; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); small inducible cytokine subfamily A (Cys-Cys), member 2; Small-inducible cytokine A2

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Gene Aliases: CCL2; GDCF-2; HC11; HSMCR30; MCAF; MCP-1; MCP1; SCYA2; SMC-CF

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UniProt ID: (Human) P13500

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Entrez Gene ID: (Human) 6347

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Molecular Function: cytokine intercellular signal molecule

Function(s)

Process(es)

protein kinase activity receptor binding chemokine activity heparin binding CCR2 chemokine receptor binding

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MCP-1 Polyclonal Antibody, Biotin, PeproTech®

MCP-1 Antibody (500-P34BT-1MG) in WB

Product Details

500-P34BT-1MG

Western Blot (WB)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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