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Invitrogen
MCU Monoclonal Antibody (CL3576)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
MCU Antibody (MA5-51968) in ICC/IF
Immunofluorescent staining of MCU in human A549 cells using the MCU monoclonal antibody (Product # MA5-24702), showing specific staining in the mitochondria in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Product Details
MA5-51968
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
CL3576
Immunogen
Recombinant Human MCU
Conjugate
Form
Liquid
Concentration
1.00 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
0
Product Specific Information
Immunogen sequence: TRQEYVYPEAR DRQYLLFFHK GAKKSRFDLE KYNQLKDAIA QAEMDLKRLR DPLQVHLPLR QIGE
Binds to an epitope located within the peptide sequence RDRQYLLFFH as determined by overlapping synthetic peptides.
Highest antigen sequence identity to the following orthologs - mouse 100%, rat 100%.
Target Information
Mitochondrial inner membrane calcium uniporter that mediates calcium uptake into mitochondria. Constitutes the pore-forming and calcium-conducting subunit of the uniporter complex (uniplex). Activity is regulated by MICU1 and MICU2. At low Ca(2+) levels MCU activity is down-regulated by MICU1 and MICU2; at higher Ca(2+) levels MICU1 increases MCU activity. Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates cell bioenergetics, cytoplasmic calcium signals and activation of cell death pathways. Involved in buffering the amplitude of systolic calcium rises in cardiomyocytes. While dispensable for baseline homeostatic cardiac function, acts as a key regulator of short-term mitochondrial calcium loading underlying a 'fight-or-flight' response during acute stress: acts by mediating a rapid increase of mitochondrial calcium in pacemaker cells. participates in mitochondrial permeability transition during ischemia-reperfusion injury. Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake. Mitochondrial calcium uptake in skeletal muscle cells is involved in muscle size in adults. Regulates synaptic vesicle endocytosis kinetics in central nerve terminal. Involved in antigen processing and presentation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Calcium uniporter protein, mitochondrial; Coiled-coil domain-containing protein 109A; HsMCU
Gene Aliases: C10orf42; CCDC109A; MCU
UniProt ID: (Human) Q8NE86
Entrez Gene ID: (Human) 90550
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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