Immunofluorescent analysis of MDC1 (green) showing staining in the cytoplasm and nucleus of C2C12 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a MDC1 polyclonal antibody (Product # PA3-840) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Actin was stained using Alexa Fluor 554 (red) and nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the C-terminal residues L(276) R K H F G I K V P T T(287) of mDIC.|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA3-840 detects mitochondrial dicarboxylate carrier protein(mDIC) in human and mouse cells.
PA3-840 has been successfully used in Western blotting and ICC/IF procedures. By Western blot it detects a 28 kDa protein representing mDIC.
The PA3-840 immunogen is a synthetic peptide corresponding to the C-terminal residues L(276) R K H F G I K V P T T(287) of mDIC.
The mitochondrial dicarboxylate carrier protein (mDIC) belongs to superfamily of mitochondrial transporters. It has been demonstrated that the expression of mDIC leads to hyperpolarization of the mitochondria, as well as succinate uptake by the mitochondria. It is highly expressed in adipose tissue, where its expression is downregulated by insulin and upregulated by free fatty acids. mDIC is also thought to play a role in glyceroneogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Predominant expression of the mitochondrial dicarboxylate carrier in white adipose tissue.
PA3-840 was used in western blot to investigate the characteristics of mitochondrial dicarboxylate carrier in mice
|Das K,Lewis RY,Combatsiaris TP,Lin Y,Shapiro L,Charron MJ,Scherer PE||The Biochemical journal (344 Pt 2:313)||1999|