Invitrogen

MDC1 Monoclonal Antibody (P2B11)

Product Image Gallery

MDC1 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of MDC1 in mouse fibroblast cell line (NIH 3T3). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with MDC1 monoclonal antibody (Product # MA5-27650) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse Alexa Fluor 488 and DAPI secondary antibody at a dilution of 1:100 (60 min at RT) and 1:5000 (5 min at RT). Magnification: 60x.

MDC1 Antibody in Western Blot (WB) — Western blot analysis of MDC1 in mouse cortex and cerebellum with 10 µg of sample. The sample was incubated with MDC1 monoclonal antibody (Product # MA5-27650) using a dilution of 1:1000 (2 hours at 4°C), and followed by Goat Anti-Mouse HRP (1 hour at RT) at a dilution of 1:2000 and ECL development (5 min at RT). Samples were arranged as follows: Lane 1: MW Ladder, Lane 2: Mouse Cortex, Lane 3: Mouse Cerebellum.

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

Assay-Dependent

Immunofluorescence (IF)

1:100

Western Blot (WB)

1:2000

Product Specifications

Species Reactivity

Bovine, Human, Mouse

Host / Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

P2B11

Immunogen

GST-tagged recombinant protein corresponding to mouse MDC1 at and around the N-terminus

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/ml

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2735265

Target Information

The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: homologue to Drosophila photoreceptor protein calphotin; mediation of DNA damage checkpoint 1; mediator of DNA damage checkpoint 1; Mediator of DNA damage checkpoint protein 1; mediator of DNA-damage checkpoint 1; Nuclear factor with BRCT domains 1; Nuclear factor with BRCT domains1

Gene Aliases: 6820401C03; AA413496; KIAA0170; MDC1; mKIAA0170; NFBD1

UniProt ID: (Human) Q14676, (Mouse) Q5PSV9

Entrez Gene ID: (Human) 9656, (Mouse) 240087

Function(s)

DNA repair cellular response to DNA damage stimulus cell cycle double-strand break repair via nonhomologous end joining protein sumoylation intra-S DNA damage checkpoint

Process(es)

protein binding protein C-terminus binding FHA domain binding