Invitrogen

MDC1 Monoclonal Antibody (P2B11)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

View all (19) MDC1 antibodies

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MDC1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Mediator of DNA damage checkpoint protein 1 was performed using 70% confluent log phase HCT 116 Nocodazole treated (200 nmol/L for 16hrs) cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with MDC1 Monoclonal Antibody (P2B11) (Product # MA5-27650) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Nuclear foci localization. Panel e represents diffused staining in untreated cells. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

MDC1 Antibody in Western Blot (WB) — Western blot analysis of MDC1 in mouse cortex and cerebellum with 10 µg of sample. The sample was incubated with MDC1 monoclonal antibody (Product # MA5-27650) using a dilution of 1:1000 (2 hours at 4°C), and followed by Goat Anti-Mouse HRP (1 hour at RT) at a dilution of 1:2000 and ECL development (5 min at RT). Samples were arranged as follows: Lane 1: MW Ladder, Lane 2: Mouse Cortex, Lane 3: Mouse Cerebellum.

MDC1 Antibody — Detection of altered subcellular localization of the target protein by cell treatment demonstrates antibody specificity. Immunofluorescence analysis using MDC1 Monoclonal Antibody (P2B11) (Product # MA5-27650), shows nuclear foci localization of MDC1 in HCT116 cells on nocodazole treatment. {TM}

Product Details

MA5-27650

Applications	Tested Dilution	Publications
Western Blot (WB)	1:2,000	-
Immunocytochemistry (ICC/IF)	1:100	-

Product Specifications
Species Reactivity	Human, Mouse, Bovine
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	P2B11
Immunogen	GST-tagged recombinant protein corresponding to mouse MDC1 at and around the N-terminus View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.4, with 50% glycerol
Contains	0.09% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2735265

Target Information

MDC1 participates in the early response to DNA damage. It is thought to be a member of the BRCT (BRCA1 C-terminus) super family of nuclear proteins. It contains an N-terminus forkhead-associated (FHA) motif, two C-terminus BRCT motifs and 13 internal repetitions of a 41 amino acid sequence.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DAAP-285E11.7; DKFZp781A0122; homologue to Drosophila photoreceptor protein calphotin; mediation of DNA damage checkpoint 1; Mediator of DNA damage checkpoint protein 1; mediator of DNA-damage checkpoint 1; MGC166888; Nuclear factor with BRCT domains 1

Gene Aliases: 6820401C03; AA413496; KIAA0170; MDC1; mKIAA0170; NFBD1

UniProt ID: (Human) Q14676, (Mouse) Q5PSV9

Entrez Gene ID: (Human) 9656, (Mouse) 240087

Function(s)

DNA repair cellular response to DNA damage stimulus cell cycle double-strand break repair via nonhomologous end joining protein sumoylation intra-S DNA damage checkpoint

Process(es)

protein binding protein C-terminus binding FHA domain binding