Invitrogen

MDC1 Monoclonal Antibody (P2B11)

Product Image Gallery

MDC1 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of MDC1 in mouse fibroblast cell line (NIH 3T3). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with MDC1 monoclonal antibody (Product # MA5-27650) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse Alexa Fluor 488 and DAPI secondary antibody at a dilution of 1:100 (60 min at RT) and 1:5000 (5 min at RT). Magnification: 60x.

MDC1 Antibody in Western Blot (WB) — Western blot analysis of MDC1 in mouse cortex and cerebellum with 10 µg of sample. The sample was incubated with MDC1 monoclonal antibody (Product # MA5-27650) using a dilution of 1:1000 (2 hours at 4°C), and followed by Goat Anti-Mouse HRP (1 hour at RT) at a dilution of 1:2000 and ECL development (5 min at RT). Samples were arranged as follows: Lane 1: MW Ladder, Lane 2: Mouse Cortex, Lane 3: Mouse Cerebellum.

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

Assay-Dependent

Immunofluorescence (IF)

1:100

Western Blot (WB)

1:2000

Product Specifications

Species

Bovine, Human, Mouse

Expression System

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

P2B11

Immunogen

GST-tagged recombinant protein corresponding to mouse MDC1 at and around the N-terminus

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2735265

Target Information

MDC1 participates in the early response to DNA damage. It is thought to be a member of the BRCT (BRCA1 C-terminus) super family of nuclear proteins. It contains an N-terminus forkhead-associated (FHA) motif, two C-terminus BRCT motifs and 13 internal repetitions of a 41 amino acid sequence.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DAAP-285E11.7; DKFZp781A0122; homologue to Drosophila photoreceptor protein calphotin; mediation of DNA damage checkpoint 1; Mediator of DNA damage checkpoint protein 1; mediator of DNA-damage checkpoint 1; MGC166888; Nuclear factor with BRCT domains 1

Gene Aliases: 6820401C03; AA413496; KIAA0170; MDC1; mKIAA0170; NFBD1

UniProt ID: (Human) Q14676, (Mouse) Q5PSV9

Entrez Gene ID: (Human) 9656, (Mouse) 240087

Function(s)

DNA repair cellular response to DNA damage stimulus cell cycle double-strand break repair via nonhomologous end joining protein sumoylation intra-S DNA damage checkpoint

Process(es)

protein binding protein C-terminus binding FHA domain binding