Western blot analysis of MDM2 using A) 30 µg Jurkat whole cell lysate (B) 30 µgRaji whole cell lysate and C) 30 µg NCI-H929 whole cell lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a MDM2 monoclonal antibody (Product # MA1-25896) at a dilution of 1:500.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Human MDM2 protein.|
|Storage buffer||0.05M sodium phosphate, pH 7.5, with 0.2% gelatin|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1 ug/ml|
|Immunofluorescence (IF)||1 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||2.5 µg/ml|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:50-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-25896 detects MDM2 from human samples.
MA1-25896 has been successfully used in immunofluorescence, immunohistochemistry (paraffin), immunoprecipitation, and Western blot procedures. Heat pretreatment is required for paraffin sections.
The MA1-25896 immunogen is human MDM2 protein.
This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.