Western blot of MDM2 in whole extracts of HEK-293 cells overexpressing human (lane 1) or mouse (lane 2) MDM2 using a MDM2 monoclonal antibody (Product # MA1-24755) at a dilution of 2 µg/mL followed by detection using a Goat anti-mouse IgG-Peroxidase and chemiluminescent substrate.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Synthetic peptide, conjugated to KLH, corresponding to residues 389-402 of human MDM2.|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-24755 detects MDM2 from human and mouse samples.
MA1-24755 has been successfully used in Western blot procedures.
The MA1-24755 immunogen is a synthetic peptide, conjugated to KLH, corresponding to residues 389-402 of human MDM2.
This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.