Immunofluorescent analysis of MDM2 (green) in HeLa cells either left untreated (left panel) or treated with 50nM Nocodazole (right panel) for 16 hours. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature and blocked with 3% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a MDM2 monoclonal antibody (Product # MA1-113) at a concentration of 1ug/ml for at least 1 hour at room temperature, washed with PBS, and incubated with a Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor 488 conjugate (Product # A28175) at a dilution of 1:400 for 30 minutes at room temperature. Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or ToxInsight Instrument at 20X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG2b|
|Storage buffer||PBS, pH 7.2-7.4, with 1mg/ml BSA, 30% glycerol|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1-5 µg/ml|
|Immunofluorescence (IF)||1 - 5 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||1 - 5 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:500|
|Immunoprecipitation (IP)||1 - 5 µg/ml|
|Western Blot (WB)||0.5-2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody recognizes the ~90 kDa (apparent MW) MDM2 protein. Also recognizes isoforms at ~57 and ~74/76 kDa.
This antibody was originally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.
This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Myxoid liposarcoma with heterologous components: dedifferentiation or metaplasia? A FISH-documented and CGH-documented case report.
MA1-113 was used in immunohistochemistry - paraffin section to characterize a primary myxoid liposarcoma with chondroid and osseous components
|Weingertner N,Neuville A,Chibon F,Ray-Coquard I,Marcellin L,Ghnassia JP||Applied immunohistochemistry and molecular morphology : AIMM (23:230)||2015|
alpha-fetoprotein expression in a dedifferentiated liposarcoma.
MA1-113 was used in immunohistochemistry - paraffin section to present a unique case of relapsing retroperitoneal dedifferentiated liposarcoma with alpha-fetoprotein ectopic production
|Bosco M,Allia E,Coindre JM,Odasso C,Pagani A,Pacchioni D||Virchows Archiv : an international journal of pathology (448:517)||2006|
Palmar atypical lipomatous tumour with spindle cell features (well-differentiated spindle cell liposarcoma): a rare neoplasm arising in an unusual anatomical location.
MA1-113 was used in immunohistochemistry - paraffin section to present the case of a patient with a rare atypical lipomatous tumor with spindle cell features
|Mentzel T,Toennissen J,Rütten A,Schaller J||Virchows Archiv : an international journal of pathology (446:300)||2005|
MDM2 and CDK4 immunohistochemical coexpression in high-grade osteosarcoma: correlation with a dedifferentiated subtype.
MA1-113 was used in immunohistochemistry (paraffin) to discuss markers that distinguish between different subtypes of osteosarcomas.
|Yoshida A,Ushiku T,Motoi T,Beppu Y,Fukayama M,Tsuda H,Shibata T||The American journal of surgical pathology (36:423)||2012|
Well-differentiated liposarcoma with low-grade osteosarcomatous component: an underrecognized variant.
MA1-113 was used in immunohistochemistry - paraffin section to use 9 cases of well-differentiated/dedifferentiated liposarcoma to characterize unappreciated features
|Yoshida A,Ushiku T,Motoi T,Shibata T,Fukayama M,Tsuda H||The American journal of surgical pathology (34:1361)||2010|
Inflammatory malignant fibrous histiocytomas and dedifferentiated liposarcomas: histological review, genomic profile, and MDM2 and CDK4 status favour a single entity.
MA1-113 was used in immunohistochemistry (frozen) to characterize dedifferentiated liposarcomas with an inflammatory malignant fibrous histiocytoma component.
|Coindre JM,Hostein I,Maire G,Derré J,Guillou L,Leroux A,Ghnassia JP,Collin F,Pedeutour F,Aurias A||The Journal of pathology (203:822)||2004|
Most malignant fibrous histiocytomas developed in the retroperitoneum are dedifferentiated liposarcomas: a review of 25 cases initially diagnosed as malignant fibrous histiocytoma.
MA1-113 was used in immunohistochemistry (paraffin) to report that most so-called malignant fibrous histiocytomas from the retroperitoneum are dedifferentiated liposarcoma.
|Coindre JM,Mariani O,Chibon F,Mairal A,De Saint Aubain Somerhausen N,Favre-Guillevin E,Bui NB,Stoeckle E,Hostein I,Aurias A||Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc (16:256)||2003|
MDM2 and CDK4 immunohistochemistry is a valuable tool in the differential diagnosis of low-grade osteosarcomas and other primary fibro-osseous lesions of the bone.
MA1-113 was used in immunohistochemistry to examine expression of MDM2-CDK4 in low-grade osteosarcomas and fibrous or fibro-osseous lesions of the bone or paraosseous soft tissue.
|Dujardin F,Binh MB,Bouvier C,Gomez-Brouchet A,Larousserie F,Muret Ad,Louis-Brennetot C,Aurias A,Coindre JM,Guillou L,Pedeutour F,Duval H,Collin C,de Pinieux G||Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc (24:624)||2011|
|Not Applicable||Not Cited||
Lipoleiomyosarcoma of the rectosigmoid colon: a unique site for a rare variant of liposarcoma.
MA1-113 was used in immunohistochemistry to discuss a rare presentation of a lipoleiomyosarcoma and review the method of pathologic diagnosis
|Nahal A,Meterissian S||American journal of clinical oncology (32:353)||2009|
|Not Applicable||Not Cited||
Sporadic desmoid tumor. An exceptional cause of cystic pancreatic lesion.
MA1-113 was used in immunohistochemistry to discuss the properties of desmoid tumors
|Amiot A,Dokmak S,Sauvanet A,Vilgrain V,Bringuier PP,Scoazec JY,Sastre X,Ruszniewski P,Bedossa P,Couvelard A||JOP : Journal of the pancreas (9:339)||2008|