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MED4 Antibody (MA5-44913) in IHC (P)
Immunohistochemistry analysis of MED4 in paraffin-embedded human small intestine tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with MED4 Monoclonal antibody (Product # MA5-44913) using a dilution of 1:200 for 30 minut... View More
Product Details
MA5-44913
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JE56-55
Immunogen
Recombinant protein within human MED4 aa 100-250.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2931369
Target Information
The Mediator is a multiprotein coactivator that is required by DNA-binding transcription factors for activation of polymerase II transcribed genes. MED4 appears to be a core Mediator subunit and is found in nearly all Mediator preparations (Sato et al., 2004).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Activator-recruited cofactor 36 kDa component; ARC36; DRIP36; FLJ10956; Mediator complex subunit 4; Mediator of RNA polymerase II transcription subunit 4; mediator of RNA polymerase II transcription, subunit 4 homolog; mediator, 34-kD subunit, homolog; p36 TRAP/SMCC/PC2 subunit; RP11-90M2.2; TRAP/SMCC/PC2 subunit p36; TRAP/SMCC/PC2 subunit p36 subunit; vitamin D receptor interacting protein; vitamin D receptor-interacting protein, 36-kD; Vitamin D3 receptor-interacting protein complex 36 kDa component
Gene Aliases: 2410046H15Rik; ARC36; DRIP36; HSPC126; MED4; TRAP36; Vdirp; VDRIP
UniProt ID: (Human) Q9NPJ6, (Rat) Q561Q8, (Mouse) Q9CQA5
Entrez Gene ID: (Human) 29079, (Rat) 306030, (Mouse) 67381
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
intracellular steroid hormone receptor signaling pathway
androgen receptor signaling pathway
positive regulation of transcription, DNA-templated
biological_process
transcription, DNA-templated
regulation of transcription, DNA-templated
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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