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|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to aa 487-507 of human MEF-2.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is brain tissue.
The myocyte enhancer factors-2 (MEF-2) family of transcription factors associate with a variety of co-repressors or co-activators to regulate development and function of T cells, neuronal cells, and muscle cells. Four distinct family members arise from alternatively splices transcripts, termed MEF-2A, MEF-2B, MEF-2C, and MEF-2D. These members bind as homo- and heterodimers to the MEF-2 site in the promoter region of affected genes. Differential regulation in the expression of the 4 transcripts implies functional distinction for each during embryogenesis and development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
ADCAD1; MADS box transcription enhancer factor 2; MADS box transcription enhancer factor 2, polypeptide A (myocyte enhancer factor 2A); mef2; myocyte-specific enhancer factor 2A; polypeptide A myocyte enhancer factor 2A; RSRFC4; RSRFC9; serum response factor-like protein 1
ADCAD1; MEF2; MEF2A; RSRFC4; RSRFC9