Invitrogen

MEIS1 Monoclonal Antibody (OTI3G8)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

View all (17) MEIS1 antibodies

Datasheet

Datasheet

Product Image Gallery

MEIS1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded human endometrium tissue. To expose target proteins, heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min. Following antigen retrieval, tissues were probed with a MEIS1 monoclonal antibody (Product # MA5-27190) at a dilution of 1:500.

MEIS1 Antibody in Western Blot (WB) — Western blot was performed using Anti-MEIS1 Monoclonal Antibody (Product # MA5-27190) and a 50kDa band corresponding to MEIS1 was observed in IMR-32, HeLa and not in Daudi which is reported negative for MEIS1. Modified whole cell extracts (1% SDS) (30 µg lysate) of IMR-32 (Lane 1), HeLa (Lane 2) and Daudi (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence Goat Anti-Mouse IgG Secondary Antibody, HRP conjugate (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752).

MEIS1 Antibody in Relative expression — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of MEIS1 was observed in IMR-32, HeLa in comparison to Daudi using Anti-MEIS1 Monoclonal Antibody (Product # MA5-27190) in Western Blot. {RE}

Product Details

Tested Applications

Dilution

Immunohistochemistry (Paraffin) (IHC (P))

1:500

Western Blot (WB)

1:500-1:2000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI3G8

Immunogen

Human recombinant protein fragment corresponding to amino acids 176-390 of MEIS1 produced in E.coli

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2724672

Target Information

MEIS1, also known as Homeobox protein Meis1, is a nuclear protein belonging to the TALE/MEIS homeobox family with a homeobox DNA-binding domain. It acts as a transcriptional regulator of PAX6 (Paired box protein Pax-6). It is a protein required for hematopoiesis, megakaryocyte lineage development and vascular patterning. Expression is seen at low levels in normal immunohepatopoietic tissues, and in a subset of myeloid leukemia cell lines, with the highest expression seen in those with a megakaryocytic-erythroid phenotype. It is also expressed at high levels in the cerebellum.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Homeobox protein Meis1; leukemogenic homolog protein; Meis homeobox 1; MEIS1; Meis1, myeloid ecotropic viral integration site 1 homolog; MGC43380; Myeloid ecotropic viral integration site 1; WUGSC:H_NH0444B04.1

Gene Aliases: C530044H18Rik; Evi8; MEIS1

UniProt ID: (Human) O00470, (Mouse) Q60954

Entrez Gene ID: (Human) 4211, (Rat) 686117, (Mouse) 17268

Molecular Function: DNA-directed RNA polymerase RNA binding protein