Invitrogen

MEIS1 Monoclonal Antibody (OTI3G8)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (21) MEIS1 antibodies

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Datasheet

Protocols

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MEIS1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded human endometrium tissue. To expose target proteins, heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min. Following antigen retrieval, tissues were probed with a MEIS1 monoclonal antibody (Product # MA5-27190) at a dilution of 1:500.

MEIS1 Antibody in Western Blot (WB) — Western blot was performed using Anti-MEIS1 Monoclonal Antibody (Product # MA5-27190) and a 50kDa band corresponding to MEIS1 was observed in IMR-32, HeLa and not in Daudi which is reported negative for MEIS1. Modified whole cell extracts (1% SDS) (30 µg lysate) of IMR-32 (Lane 1), HeLa (Lane 2) and Daudi (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence Goat Anti-Mouse IgG Secondary Antibody, HRP conjugate (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752).

MEIS1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of MEIS1 was observed in IMR-32, HeLa in comparison to Daudi using Anti-MEIS1 Monoclonal Antibody (Product # MA5-27190) in Western Blot. {RE}

Product Details

MA5-27190

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:500	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	OTI3G8
Immunogen	Human recombinant protein fragment corresponding to amino acids 176-390 of MEIS1 produced in E.coli View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2724672

Target Information

Homeobox genes, of which the most well-characterized category is represented by the HOX genes, play a crucial role in normal development. In addition, several homeoproteins are involved in neoplasia. This gene encodes a homeobox protein belonging to the TALE family of homeodomain-containing proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Homeobox protein Meis1; leukemogenic homolog protein; Meis1, myeloid ecotropic viral integration site 1 homolog; MGC43380; Myeloid ecotropic viral integration site 1; WUGSC:H_NH0444B04.1

Gene Aliases: C530044H18Rik; Evi8; MEIS1

UniProt ID: (Human) O00470, (Mouse) Q60954

Entrez Gene ID: (Human) 4211, (Rat) 686117, (Mouse) 17268

Molecular Function: homeodomain transcription factor