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|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide: 15 amino acids surrounding 283 of human MEK1|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2, with 1% BSA, 50% glycerol|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||20-40 µg/ml|
|Immunoprecipitation (IP)||4-8 µg/ml|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-21730 detects MEK1 in Human, Mouse and Rat samples.
PA1-21730 has been successfully used in Immunohistochemistry (Frozen), Immunoprecipitation and Western Blot procedures.
PA1-21730 immunogen corresponds to Synthetic peptide: 15 amino acids surrounding 283 of human MEK1.
MAP kinase kinase 1 (Mek 1), also known as mitogen activated protein kinase kinase, MAP kinase kinase, ERK kinase 1 or microtubule-associated protein 2 kinase, is a 43 kDa dual-specificity protein kinase that phosphorylates threonine and tyrosine residues on MAP kinases such as Erk 1 and 2. Phosphorylation by Mek1 increases Erk's ability to phosphorylate microtubule-associated proteins and myelin basic proteins. Mek 1 is involved in a diverse array of cellular processes such as stress-activated response, apoptosis, cytokine-induced cell proliferation, and DNA recombination during meiosis. Mek 1 is activated by phosphorylation on serine 218 and 222 by serine-threonine kinase, Raf-1, which is part of the p21ras signal transduction pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAP kinase kinase 1; MAP kinase/Erk kinase 1; MAPK/ERK kinase 1; MAPKK 1; MAPKK1; MEK 1; mitogen activated protein kinase kinase 1; mitogen-activated protein kinase kinase 1; MKK1; PRKMK1; protein kinase, mitogen activated, kinase 1, p45; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
CFC3; MAP2K1; MAPKK1; MEK1; MEKK1; MKK1; PRKMK1