|Flow Cytometry (Flow)||2-4µg/10^6 cells|
|Immunocytochemistry (ICC)||5 ug/ml|
|Immunofluorescence (IF)||5 ug/ml|
|Western Blot (WB)||0.5-1µg/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Contains||0.09% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
This antibody is predicted to react with bovine, chicken, mouse and rat based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
MEK2 belongs to the family of mitogen-activated protein kinase kinases that phosphorylate threonine and tyrosine residues within the activation loop of their MAP kinase substrates. The ERK1/2 MAP kinase signaling pathway is an integral part of cell proliferation control and is frequently activated in human colorectal cancer. Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221 and is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Dual specificity mitogen-activated protein kinase kinase 2; ERK activator kinase 2; MAP kinase kinase 2; MAPK/ERK kinase 2; MAPKK 2; MEK 2; mitogen-activated protein kinase kinase 2, p45
Gene Aliases: CFC4; MAP2K2; MAPKK2; MEK2; MKK2; PRKMK2
UniProt ID: (Human) P36507
Entrez Gene ID: (Human) 5605
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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