Immunofluorescent analysis of MEK2 in HeLa cells using a MEK2 recombinant rabbit monoclonal antibody (Product # 700829) at a dilution of 10ug/ml followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody at a dilution of 1:1000. Cells were counterstained with Alexa Fluor 568 Phalliodin (Product # A12380) and treated with SlowFade Gold with DAPI (Product # S36936).
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||2-4µg/10^6 cells|
|Immunocytochemistry (ICC)||5 ug/ml|
|Immunofluorescence (IF)||5 ug/ml|
|Western Blot (WB)||0.5-1µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with bovine, chicken, mouse and rat based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
MEK2 belongs to the family of mitogen-activated protein kinase kinases that phosphorylate threonine and tyrosine residues within the activation loop of their MAP kinase substrates. The ERK1/2 MAP kinase signaling pathway is an integral part of cell proliferation control and is frequently activated in human colorectal cancer. Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221 and is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.