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Immunofluorescent analysis of U251 cells using a MEK2 polyclonal antibody (Product # PA5-15167). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with a MEK2 polyclonal antibody (Product # PA5-15167) (1:25, 1 hr at 37°C). Primary antibody was detected with fluor-conjugated donkey anti-rabbit secondary antibody (green) at 1:400 dilution for 50 min at 37°C). Actin filaments have been labeled with dye-conjugated phalloidin (red).
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic peptide between 262-292 amino acids from the central region of human MEK2 (MAP2K2)|
|Purification||Antigen affinity chromatography|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MAP2K2 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. The inhibition or degradation of this kinase is found to be involved in the pathogenesis of Yersinia and anthrax.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Dual specificity mitogen-activated protein kinase kinase 2; ERK activator kinase 2; MAP kinase kinase 2; MAP kinase/Erk kinase; MAPK/ERK kinase 2; MAPKK 2; MEK 2; mitogen activated protein kinase kinase 2; mitogen-activated protein kinase kinase 2, p45; protein kinase, mitogen activated, kinase 2, p45
AA589381; CFC4; MAP2K2; MAPKK2; MEK2; MK2; MKK2; PRKMK2