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Invitrogen
MEK3/MEK6 Recombinant Rabbit Monoclonal Antibody (ARC2356)
FIGURE: 1 / 2
MEK3/MEK6 Antibody (MA5-38026) in WB
Western blot analysis of MEK3/MEK6 in extracts of various cell lines. Samples were incubated with MEK3/MEK6 Monoclonal antibody (Product # MA5-38026) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
MA5-38026
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
ARC2356
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 248-347 of human MEK3/MEK6 (P46734)
Conjugate
Unconjugated
Form
Liquid
Concentration
1.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2897944
Product Specific Information
Positive Samples: HeLa, HT-1080, NIH/3T3, RD, Mouse skeletal muscle, Mouse heart, Rat skeletal muscle, Rat heart
Immunogen sequence: KSDVWSLGIT MIEMAILRFP YESWGTPFQQ LKQVVEEPSP QLPADRFSPE FVDFTAQCLR KNPAERMSYL ELMEHPFFTL HKTKKTDIAA FVKEILGEDS
Target Information
This gene encodes a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases, act as an integration point for multiple biochemical signals. This protein phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Dual specificity mitogen-activated protein kinase kinase 3; Dual specificity mitogen-activated protein kinase kinase 6; MAP kinase kinase 3; MAP kinase kinase 6; MAPK/ERK kinase 3; MAPK/ERK kinase 6; MAPKK 3; MAPKK 6; MEK 3; MEK 6; mitogen activated protein kinase kinase 3; mitogen activated protein kinase kinase 6; OTTHUMP00000166044; protein kinase, mitogen activated, kinase 6; protein kinase, mitogen-activated, kinase 3; protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6); SAPK kinase 2; SAPK kinase 3; SAPKK3; Stress-activated protein kinase kinase 2; Stress-activated protein kinase kinase 3
Gene Aliases: AW212142; MAP2K3; MAP2K6; MAPKK3; MAPKK6; MEK3; MEK6; MKK3; MKK6; mMKK3b; PRKMK3; PRKMK6; SAPKK-2; SAPKK-3; SAPKK2; SAPKK3; SKK2; SKK3
UniProt ID: (Human) P46734, (Human) P52564, (Mouse) O09110, (Mouse) P70236
Entrez Gene ID: (Human) 5606, (Human) 5608, (Mouse) 26397, (Rat) 303200, (Mouse) 26399, (Rat) 114495
receptor signaling protein serine/threonine kinase activity
MAP kinase kinase activity
protein tyrosine kinase activity
protein binding
ATP binding
protein kinase binding
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
MAPK cascade
activation of MAPK activity
inflammatory response
signal transduction
peptidyl-tyrosine phosphorylation
proteolysis in other organism
regulation of cytokine biosynthetic process
positive regulation of protein kinase activity
positive regulation of transcription, DNA-templated
cardiac muscle contraction
response to ischemia
transcription, DNA-templated
regulation of transcription, DNA-templated
apoptotic process
DNA damage induced protein phosphorylation
cell cycle arrest
ovulation cycle process
positive regulation of prostaglandin secretion
response to drug
positive regulation of apoptotic process
positive regulation of nitric-oxide synthase biosynthetic process
nucleotide-binding oligomerization domain containing signaling pathway
cellular response to sorbitol
protein phosphorylation
regulation of mitotic cell cycle
phosphorylation
stress-activated protein kinase signaling cascade
regulation of apoptotic process
positive regulation of protein phosphorylation
heart development
cellular response to lipopolysaccharide
regulation of signal transduction
intracellular signal transduction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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