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|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Synthesized peptide of human MELK(amino acids: 637-651:C-VYKRLVEDILSSCKV).|
|Storage buffer||PBS with 0.5% proprietary stabilizer|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:200 - 1:400|
|Immunocytochemistry (ICC)||1:200 - 1:1000|
|Immunofluorescence (IF)||1:200 - 1:1000|
|Immunohistochemistry (IHC)||1:200 - 1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-17120 targets MELK in FACS, ICC, IHC, and IF applications and shows reactivity with Human samples.
The MA5-17120 immunogen is synthesized peptide of human MELK(amino acids: 637-651:C-VYKRLVEDILSSCKV).
MA5-17120 detects MELK which has a predicted molecular weight of approximately 74.6kDa.
Maternal embryonic leucine-zipper kinase (MELK) is a key regulator of survival of stemlike GBM cells in vitro. MELK expression is increased in breast cancer tissue and this increase is also associated with poor patient survival, as predicted for a candidate oncogene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
pEg3 kinase; protein kinase Eg3; protein kinase PK38; tyrosine-protein kinase MELK
HPK38; KIAA0175; MELK