Proteintech

MEST Monoclonal Antibody (1A4A1)

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MEST Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed BxPC-3 cells using 60263-1-IG (MEST antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

MEST Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 60263-1-IG ( MEST Antibody) at dilution of 1:400 (under 40x lens).

MEST Antibody in Western Blot (WB) — PC-3 cells were subjected to SDS PAGE followed by western blot with 60263-1-IG (MEST antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Product Details

60263-1-IG

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:50-1:500	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Clone	1A4A1
Immunogen	MEST Fusion Protein Ag11929 (1-234 aa encoded by BC011908) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	2 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MVRRDRLRR MREWWVQVGL LAVPLLAAYL HIPPPQLSPA LHSWKSSGKF FTYKGLRIFY QDSVGVVGSP EIVVLLHGFP TSSYDWYKIW EGLTLRFHRV IALDFLGFGF SDKPRPHHYS IFEQASIVEA LLRHLGLQNR RINLLSHDYG DIVAQELLYR YKQNRSGRLT IKSLCLSNGG IFPETHRPLL LQKLLKDGGV LSPILTRLMN FFVFSRGLTP VFGPYTRPSE SELWD (1-234 aa encoded by BC011908)

Target Information

This gene encodes a member of the alpha/beta hydrolase superfamily. It is imprinted, exhibiting preferential expression from the paternal allele in fetal tissues, and isoform-specific imprinting in lymphocytes. The loss of imprinting of this gene has been linked to certain types of cancer and may be due to promotor switching. The encoded protein may play a role in development. Alternatively spliced transcript variants encoding multiple isoforms have been identified for this gene. Pseudogenes of this gene are located on the short arm of chromosomes 3 and 4, and the long arm of chromosomes 6 and 15.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DKFZp686L18234; Mesoderm-specific transcript homolog protein; Mesoderm-specific transcript protein; MGC111102; MGC8703; Paternally-expressed gene 1 protein

Gene Aliases: 121a; AA408879; AI256745; MEST; PEG1

UniProt ID: (Human) Q5EB52, (Mouse) Q07646

Entrez Gene ID: (Human) 4232, (Mouse) 17294

Molecular Function: serine protease