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METAP2 Antibody (MA5-38071) in WB
Western blot analysis of METAP2 in extracts of various cell lines. Samples were incubated with METAP2 Monoclonal antibody (Product # MA5-38071) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
MA5-38071
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
ARC2022
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 380-478 of human METAP2 (NP_0068291)
Conjugate
Form
Liquid
Concentration
0.62 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2897989
Product Specific Information
Positive Samples: Rat testis, DU145, HT-1080, NIH/3T3, C6, Mouse testis
Immunogen sequence: CSHYMKNFDV GHVPIRLPRT KHLLNVINEN FGTLAFCRRW LDRLGESKYL MALKNLCDLG IVDPYPPLCD IKGSYTAQFE HTILLRPTCK EVVSRGDDY
Target Information
The ADP-ribosylation factor (Arf) family are highly conserved members of the Ras superfamily of regulatory GTP-binding proteins. Arf proteins participate in routing of intracellular proteins to and within the Golgi complex. Cellular functions include maintenance of organelle integrity, coat protein assembly, as an activator of phospholipase D. The Arf family is divided functionally into the Arf and the Arf-like (Arl) proteins. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6) and members of each class share a common gene organization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: eIF-2-associated p67; eIF-2-associated p67 homolog; initiation factor 2 associated 67 kDa protein; Initiation factor 2-associated 67 kDa glycoprotein; initiation factor 2-associated protein (p67); MAP 2; metAP 2; Methionine aminopeptidase 2; p67; p67eIF2; Peptidase M; peptidase M 2; testicular tissue protein Li 17
Gene Aliases: 4930584B20Rik; A930035J23Rik; AI047573; AL024412; Amp2; AU014659; MAP2; METAP2; MNPEP; p67; P67EIF2
UniProt ID: (Human) P50579, (Mouse) O08663, (Rat) P38062
Entrez Gene ID: (Human) 10988, (Mouse) 56307, (Rat) 64370
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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