MA5-13503 targets MGMT (O-methylguanine-DNA methyltransferase) in FACS, IHC (P), ICC/IF and WB applications and shows reactivity with Human samples.
The MA5-13503 immunogen is purified recombinant human MGMT protein.
Cancer chemotherapeutic alkylating agents (e.g. BCNU,) act by inducing formation of lethal cross-links at the O-alkylguanine position in DNA. MGMT transfers alkyl adducts from the O-position of guanine in DNA (prior to cross-link formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible; hence the level of MGMT in a cell is directly proportional to the level of DNA-damage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely be drug-resistant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 6-O-methylguanine-DNA methyltransferase; methylated DNA protein cysteine methyltransferase; Methylated-DNA--protein-cysteine methyltransferase; methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase; O6-methylguanine-DNA methyltransferase
Gene Aliases: MGMT
UniProt ID: (Human) P16455
Entrez Gene ID: (Human) 4255