Invitrogen

MHC Class I (H-2Kb) Monoclonal Antibody (AF6-88.5.5.3), FITC, eBioscience™

Product Image Gallery

MHC Class I (H-2Kb) Antibody in Flow Cytometry (Flow) — Staining of BALB/c (left) and C57Bl/6 (right) splenocytes with 0.5 µg of Mouse IgG2a K Isotype Control FITC (Product # 11-4724-42) (blue histogram) or 0.5 µg of Anti-Mouse MHC Class I (H-2Kb) FITC (purple histogram). Total viable cells were used for analysis.

Product Details

11-5958-82

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	1 µg/test	View 14 publications 14 publications
Immunocytochemistry (ICC/IF)	-	View 2 publications 2 publications

Product Specifications
Species Reactivity	Mouse
Published species	Human, Mouse
Host/Isotype	Mouse / IgG2a, kappa
Recommended Isotype Control	Mouse IgG2a kappa Isotype Control (eBM2a), FITC, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	AF6-88.5.5.3
Conjugate	FITC Alexa Fluor 700 APC APC-eFluor 780 Biotin eFluor 450 NovaFluor Blue 510 NovaFluor Blue 610-70S NovaFluor Blue 660-120S NovaFluor Red 700 PE PE-Cyanine7 PE-eFluor 610 PerCP-eFluor 710 Super Bright 436 Super Bright 600 Super Bright 702
Excitation/Emission Max	498/517 nm View spectra
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_11149502

Product Specific Information

Description: This AF6-88.5.5.3 monoclonal antibody reacts with the H-2Kb MHC class I alloantigen. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8. Reactivity to other haplotypes (e.g. d, f, j, k, p, q, r, s, u, and v) has not been observed.

Applications Reported: This AF6-88.5.5.3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This AF6-88.5.5.3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

Mesenchymal stem cell exosome-derived miR-223 alleviates acute graft-versus-host disease via reducing the migration of donor T cells.

Stem cell research & therapy

Liu W,Zhou N,Liu Y,Zhang W,Li X,Wang Y,Zheng R,Zhang Y

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 11-5958-82) in Flow Cytometry

Fri Feb 26 00:00:00 EST 2021

Intravenous injection of the oncolytic virus M1 awakens antitumor T cells and overcomes resistance to checkpoint blockade.

Cell death & disease

Liu Y,Cai J,Liu W,Lin Y,Guo L,Liu X,Qin Z,Xu C,Zhang Y,Su X,Deng K,Yan G,Liang J

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 11-5958-82) in Flow Cytometry

Sat Dec 12 00:00:00 EST 2020

Small Molecule Enhancers of Endosome-to-Cytosol Import Augment Anti-tumor Immunity.

Cell reports

Kozik P,Gros M,Itzhak DN,Joannas L,Heurtebise-Chrétien S,Krawczyk PA,Rodríguez-Silvestre P,Alloatti A,Magalhaes JG,Del Nery E,Borner GHH,Amigorena S

11-5958 was used in Flow cytometry/Cell sorting to identify 37 enhancers of antigen import from endolysosomes into the cytosol from screening 700 US Food and Drug Administration (FDA)-approved drugs and reveal their mechanism of action.

Tue Jul 14 00:00:00 EDT 2020

Platelet-derived β2m regulates age related monocyte/macrophage functions.

Aging

Hilt ZT,Ture SK,Mohan A,Arne A,Morrell CN

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 11-5958-82) in Flow Cytometry

Wed Dec 18 00:00:00 EST 2019

CXCL14 suppresses human papillomavirus-associated head and neck cancer through antigen-specific CD8⁺ T-cell responses by upregulating MHC-I expression.

Oncogene

Westrich JA,Vermeer DW,Silva A,Bonney S,Berger JN,Cicchini L,Greer RO,Song JI,Raben D,Slansky JE,Lee JH,Spanos WC,Pyeon D

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 11-5958-82) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2019

The CARD9-Associated C-Type Lectin, Mincle, Recognizes La Crosse Virus (LACV) but Plays a Limited Role in Early Antiviral Responses against LACV.

Viruses

Monteiro JT,Schön K,Ebbecke T,Goethe R,Ruland J,Baumgärtner W,Becker SC,Lepenies B

11-5958-82 was used in Flow Cytometry to investigate the role of additional C-type lectin receptors/La Crosse virus interactions.

Tue Mar 26 00:00:00 EDT 2019

Genetic and phenotypic characterization of the novel mouse substrain C57BL/6N Korl with increased body weight.

Scientific reports

Choi KM,Jung J,Cho YM,Kim K,Kim MG,Kim J,Kim H,Shin HJ,Kim HD,Chung ST,Cho S,Chung MW

11-5958 was used in Immunocytochemistry-immunoflourescence to investigate the underlying genetic profiles of a newly developed mouse strain, C57BL/6NKorl.

Fri Oct 27 00:00:00 EDT 2017

Critical role for Sec22b-dependent antigen cross-presentation in antitumor immunity.

The Journal of experimental medicine

Alloatti A,Rookhuizen DC,Joannas L,Carpier JM,Iborra S,Magalhaes JG,Yatim N,Kozik P,Sancho D,Albert ML,Amigorena S

11-5958 was used in Flow cytometry/Cell sorting to determine that Sec22b-dependent cross-presentation in dendritic cells is required to initiate CD8 T cell responses to dead cells, and induce effective anti-tumour immune responses during anti-PD-1 treatment.

Mon Aug 07 00:00:00 EDT 2017

Sec61 blockade by mycolactone inhibits antigen cross-presentation independently of endosome-to-cytosol export.

Proceedings of the National Academy of Sciences of the United States of America

Grotzke JE,Kozik P,Morel JD,Impens F,Pietrosemoli N,Cresswell P,Amigorena S,Demangel C

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 11-5958-82) in Flow Cytometry

Tue Jul 18 00:00:00 EDT 2017

Reprogramming MHC specificity by CRISPR-Cas9-assisted cassette exchange.

Scientific reports

Kelton W,Waindok AC,Pesch T,Pogson M,Ford K,Parola C,Reddy ST

11-5958 was used in Immunocytochemistry to demonstrate a proof-of-concept for reprogramming MHC-specificity by performing CRISPR-Cas9-assisted cassette exchange.

Tue Apr 04 00:00:00 EDT 2017

Deletion of Wiskott-Aldrich syndrome protein triggers Rac2 activity and increased cross-presentation by dendritic cells.

Nature communications

Baptista MA,Keszei M,Oliveira M,Sunahara KK,Andersson J,Dahlberg CI,Worth AJ,Liedén A,Kuo IC,Wallin RP,Snapper SB,Eidsmo L,Scheynius A,Karlsson MC,Bouma G,Burns SO,Forsell MN,Thrasher AJ,Nylén S,Westerberg LS

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 11-5958-82) in Flow Cytometry

Mon Jul 18 00:00:00 EDT 2016

Metabolic reprogramming of alloantigen-activated T cells after hematopoietic cell transplantation.

The Journal of clinical investigation

Nguyen HD,Chatterjee S,Haarberg KM,Wu Y,Bastian D,Heinrichs J,Fu J,Daenthanasanmak A,Schutt S,Shrestha S,Liu C,Wang H,Chi H,Mehrotra S,Yu XZ

11-5958 was used in Flow cytometry/Cell sorting to study the metabolic reprogramming of donor T cells following allogeneic haematopoietic cell transplantation.

Fri Apr 01 00:00:00 EDT 2016

Anti-angiogenic efficacy of 5'-triphosphate siRNA combining VEGF silencing and RIG-I activation in NSCLCs.

Oncotarget

Yuan D,Xia M,Meng G,Xu C,Song Y,Wei J

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 11-5958-82) in Flow Cytometry

Tue Oct 06 00:00:00 EDT 2015

Immune selection of tumor cells in TCR β-chain transgenic mice.

Journal of immunotoxicology

Silaeva YY,Grinenko TS,Vagida MS,Kalinina AA,Khromykh LM,Kazansky DB

11-5958 was used in Flow cytometry/Cell sorting to compare the ability of WT and transgenic, immune-compromised mice to reject EL4 thymoma cells.

Wed Oct 01 00:00:00 EDT 2014

Oxidized lipids block antigen cross-presentation by dendritic cells in cancer.

Journal of immunology (Baltimore, Md. : 1950)

Ramakrishnan R,Tyurin VA,Tuyrin VA,Veglia F,Condamine T,Amoscato A,Mohammadyani D,Johnson JJ,Zhang LM,Klein-Seetharaman J,Celis E,Kagan VE,Gabrilovich DI

11-5958 was used in Flow cytometry/Cell sorting to reveal a novel role for oxidised lipids in the regulation of cross-presentation.

Sat Mar 15 00:00:00 EDT 2014

c-Rel is an essential transcription factor for the development of acute graft-versus-host disease in mice.

European journal of immunology

Yu Y,Wang D,Kaosaard K,Liu C,Fu J,Haarberg K,Anasetti C,Beg AA,Yu XZ

11-5958 was used in Flow cytometry/Cell sorting to investigate the role of c-Rel in transplant tolerance and graft-versus-host disease (GVHD), showing that it is an essential transcription factor for the development of acute GVHD.

Sun Sep 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: class I major histocompatibility antigen H-2Kd; H-2 class I histocompatibility antigen, K-B alpha chain; H-2 class I histocompatibility antigen, K-D alpha chain; H-2 class I histocompatibility antigen, K-K alpha chain; H-2 class I histocompatibility antigen, K-Q alpha chain; H-2 class I histocompatibility antigen, K-W28 alpha chain; H-2K(B); H-2K(K); H-2K(Q); MHC class I antigen; MHC class I H2-K-b-alpha-2 cell surface glycoprotein; MHC class I heavy chain H2-K; MHC H2-K transplantation antigen

Gene Aliases: H-2K; H-2K(d); H2-K; H2-K1; K-f

UniProt ID: (Mouse) P01901

Entrez Gene ID: (Mouse) 14972

Molecular Function: major histocompatibility complex protein

Function(s)

positive regulation of T cell mediated cytotoxicity immune system process antigen processing and presentation of peptide antigen via MHC class I antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent immune response antigen processing and presentation antigen processing and presentation of exogenous peptide antigen via MHC class I defense response to bacterium inner ear development

Process(es)

receptor binding protein binding beta-2-microglobulin binding peptide binding peptide antigen binding T cell receptor binding poly(A) RNA binding TAP binding protein heterodimerization activity

MHC Class I (H-2Kb) Monoclonal Antibody (AF6-88.5.5.3), FITC, eBioscience™

Product Details

11-5958-82

Flow Cytometry (Flow)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

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