Invitrogen

MHC Class I (H-2Kb) Monoclonal Antibody (AF6-88.5.5.3), PE, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

8 Published Figures

21 References

View all (17) MHC Class I (H-2Kb) antibodies

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Protocols

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MHC Class I (H-2Kb) Antibody in Flow Cytometry (Flow) — C57BL/6 (left) or BALB/c (right) mouse splenocytes were stained with 0.125 µg of Mouse IgG2a kappa Isotype Control, PE (Product # 12-4724-82) (blue histogram) or 0.125 µg of MHC Class I (H-2Kb), PE (purple histogram). Total viable cells were used for analysis.

MHC Class I (H-2Kb) Antibody — Fig. 4. Open in a separate window Prolonged inhibition of Sec61 with ML diminishes T-cell activation capacity of MutuDCs. ( A ) Schematic representation of the assay used in B . ( B ) MutuDCs were treated for 24 h with 100 nM ML, reseeded, and incubated with OVA or OVA 257-264 peptide for 5 h. The efficiency of T-cell activation was quantified as in Fig. 1 F . ( C ) MutuDCs were treated with ML for 24 h, and the export assay was performed as described in Fig. 1 B and C . ( D ) Volcano plot showing statistical significance vs. fold change differences for each protein identified in MutuDCs treated with 100 nM ML or vehicle control for 24 h. ( E ) Pie charts depicting the proportion of down-regulated, up-regulated, or not modulated proteins among Sec61 substrates and all other identified proteins (not Sec61 substrates). **** P < 0.0001, Fisher exact test comparing the proportion of down-regulation in Sec61 substrates and all other identified proteins. ( F ) FACS analysis of MHC-I and MHC-II surface expression by MutuDCs treated with increasing doses of ML for 6, 24, and 48 h. For B , C , and F , representative data are from three independent experiments. {TM}

Product Details

12-5958-82

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	0.25 µg/test	View 21 publications 21 publications

Product Specifications
Species Reactivity	Mouse
Published species	Human, Mouse
Host/Isotype	Mouse / IgG2a, kappa
Recommended Isotype Control	Mouse IgG2a kappa Isotype Control (eBM2a), PE, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	AF6-88.5.5.3
Conjugate	PE Alexa Fluor 700 APC APC-eFluor 780 Biotin eFluor 450 FITC NovaFluor Blue 510 NovaFluor Blue 610-70S NovaFluor Blue 660-120S NovaFluor Red 700 PE-Cyanine7 PE-eFluor 610 PerCP-eFluor 710 Super Bright 436 Super Bright 600 Super Bright 702
Excitation/Emission Max	565/576 nm View spectra
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_10598797

Product Specific Information

Description: This AF6-88.5.5.3 monoclonal antibody reacts with the H-2Kb MHC class I alloantigen. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8. Reactivity to other haplotypes (e.g. d, f, j, k, p, q, r, s, u, and v) has not been observed.

Applications Reported: This AF6-88.5.5.3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This AF6-88.5.5.3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

Mesenchymal stem cell exosome-derived miR-223 alleviates acute graft-versus-host disease via reducing the migration of donor T cells.

Stem cell research & therapy

Liu W,Zhou N,Liu Y,Zhang W,Li X,Wang Y,Zheng R,Zhang Y

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 12-5958-82) in Flow Cytometry

Fri Feb 26 00:00:00 EST 2021

Intravenous injection of the oncolytic virus M1 awakens antitumor T cells and overcomes resistance to checkpoint blockade.

Cell death & disease

Liu Y,Cai J,Liu W,Lin Y,Guo L,Liu X,Qin Z,Xu C,Zhang Y,Su X,Deng K,Yan G,Liang J

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 12-5958-82) in Flow Cytometry

Sat Dec 12 00:00:00 EST 2020

Senolytics prevent mt-DNA-induced inflammation and promote the survival of aged organs following transplantation.

Nature communications

Iske J,Seyda M,Heinbokel T,Maenosono R,Minami K,Nian Y,Quante M,Falk CS,Azuma H,Martin F,Passos JF,Niemann CU,Tchkonia T,Kirkland JL,Elkhal A,Tullius SG

12-5958 was used in Flow cytometry/Cell sorting to show that cell-free mitochondrial DNA (cf-mt-DNA) released by senescent cells accumulates with aging and augments immunogenicity.

Thu Aug 27 00:00:00 EDT 2020

Platelet-derived β2m regulates age related monocyte/macrophage functions.

Aging

Hilt ZT,Ture SK,Mohan A,Arne A,Morrell CN

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 12-5958-82) in Flow Cytometry

Wed Dec 18 00:00:00 EST 2019

CXCL14 suppresses human papillomavirus-associated head and neck cancer through antigen-specific CD8⁺ T-cell responses by upregulating MHC-I expression.

Oncogene

Westrich JA,Vermeer DW,Silva A,Bonney S,Berger JN,Cicchini L,Greer RO,Song JI,Raben D,Slansky JE,Lee JH,Spanos WC,Pyeon D

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 12-5958-82) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2019

Persistence of Integrase-Deficient Lentiviral Vectors Correlates with the Induction of STING-Independent CD8⁺ T Cell Responses.

Cell reports

Cousin C,Oberkampf M,Felix T,Rosenbaum P,Weil R,Fabrega S,Morante V,Negri D,Cara A,Dadaglio G,Leclerc C

12-5958 was used in Flow cytometry/Cell sorting to demonstrate that integrase-deficient lentiviral vectors can trigger persistent adaptive response by the mediation of a weak and transient innate response alongside the persistence of the vector in tissues.

Tue Jan 29 00:00:00 EST 2019

Oncolysis with DTT-205 and DTT-304 generates immunological memory in cured animals.

Cell death & disease

Zhou H,Mondragón L,Xie W,Mauseth B,Leduc M,Sauvat A,Gomes-da-Silva LC,Forveille S,Iribarren K,Souquere S,Bezu L,Liu P,Zhao L,Zitvogel L,Sveinbjørnsson B,Eksteen JJ,Rekdal Ø,Kepp O,Kroemer G

12-5958 was used in Flow cytometry to show that DTT-205 and DTT-304 both have the capacity to induce immunotherapeutic oncolysis.

Tue Oct 23 00:00:00 EDT 2018

Proteomics Reveals Scope of Mycolactone-mediated Sec61 Blockade and Distinctive Stress Signature.

Molecular & cellular proteomics : MCP

Morel JD,Paatero AO,Wei J,Yewdell JW,Guenin-Macé L,Van Haver D,Impens F,Pietrosemoli N,Paavilainen VO,Demangel C

12-5958 was used in Flow cytometry/Cell sorting to use quantitative proteomics to delineate the direct and indirect effects of mycolactone-mediated Sec61 blockade in living cells.

Sat Sep 01 00:00:00 EDT 2018

Downregulation of Membrane Trafficking Proteins and Lactate Conditioning Determine Loss of Dendritic Cell Function in Lung Cancer.

Cancer research

Caronni N,Simoncello F,Stafetta F,Guarnaccia C,Ruiz-Moreno JS,Opitz B,Galli T,Proux-Gillardeaux V,Benvenuti F

12-5958 was used in Flow cytometry/Cell sorting to investigate the origin of dendritic cell dysfunction in a model of lung cancer and identify intrinsic mechanisms and extrinsic factors determining suppression.

Sun Apr 01 00:00:00 EDT 2018

Sec61 blockade by mycolactone inhibits antigen cross-presentation independently of endosome-to-cytosol export.

Proceedings of the National Academy of Sciences of the United States of America

Grotzke JE,Kozik P,Morel JD,Impens F,Pietrosemoli N,Cresswell P,Amigorena S,Demangel C

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 12-5958-82) in Flow Cytometry

Tue Jul 18 00:00:00 EDT 2017

CD4+ T Cells Are as Protective as CD8+ T Cells against Rickettsia typhi Infection by Activating Macrophage Bactericidal Activity.

PLoS neglected tropical diseases

Moderzynski K,Papp S,Rauch J,Heine L,Kuehl S,Richardt U,Fleischer B,Osterloh A

12-5958 was used in Flow cytometry/Cell sorting to demonstrate that CD4+ T cells are as protective as CD8+ T cells against R. typhi, provided that CD4+ TH1 effector cells are present in time to support bactericidal activity of phagocytes via the release of IFNγ and other factors.

Tue Nov 01 00:00:00 EDT 2016

Deletion of Wiskott-Aldrich syndrome protein triggers Rac2 activity and increased cross-presentation by dendritic cells.

Nature communications

Baptista MA,Keszei M,Oliveira M,Sunahara KK,Andersson J,Dahlberg CI,Worth AJ,Liedén A,Kuo IC,Wallin RP,Snapper SB,Eidsmo L,Scheynius A,Karlsson MC,Bouma G,Burns SO,Forsell MN,Thrasher AJ,Nylén S,Westerberg LS

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 12-5958-82) in Flow Cytometry

Mon Jul 18 00:00:00 EDT 2016

Favorable alteration of tumor microenvironment by immunomodulatory cytokines for efficient T-cell therapy in solid tumors.

PloS one

Tähtinen S,Kaikkonen S,Merisalo-Soikkeli M,Grönberg-Vähä-Koskela S,Kanerva A,Parviainen S,Vähä-Koskela M,Hemminki A

12-5958 was used in Flow cytometry/Cell sorting to alter the tumor microenvironment using immunomodulatory cytokines for efficient T-cell therapy in solid tumors.

Thu Apr 28 00:00:00 EDT 2016

Anti-angiogenic efficacy of 5'-triphosphate siRNA combining VEGF silencing and RIG-I activation in NSCLCs.

Oncotarget

Yuan D,Xia M,Meng G,Xu C,Song Y,Wei J

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 12-5958-82) in Flow Cytometry

Tue Oct 06 00:00:00 EDT 2015

Microfluidic squeezing for intracellular antigen loading in polyclonal B-cells as cellular vaccines.

Scientific reports

Szeto GL,Van Egeren D,Worku H,Sharei A,Alejandro B,Park C,Frew K,Brefo M,Mao S,Heimann M,Langer R,Jensen K,Irvine DJ

12-5958 was used in Flow cytometry/Cell sorting to demonstrate proof-of-concept for mechano-poration as an enabling technology for loading B-cells with antigens to prime specific CD8(+)T-cells.

Fri May 22 00:00:00 EDT 2015

Recurrence of melanoma following T cell treatment: continued antigen expression in a tumor that evades T cell recruitment.

Molecular therapy : the journal of the American Society of Gene Therapy

Straetemans T,Berrevoets C,Coccoris M,Treffers-Westerlaken E,Wijers R,Cole DK,Dardalhon V,Sewell AK,Taylor N,Verweij J,Debets R

12-5958 was used in Flow cytometry/Cell sorting to support the concept to target the tumor milieu to aid T cell therapy in limiting tumor relapse.

Sun Feb 01 00:00:00 EST 2015

c-Rel is an essential transcription factor for the development of acute graft-versus-host disease in mice.

European journal of immunology

Yu Y,Wang D,Kaosaard K,Liu C,Fu J,Haarberg K,Anasetti C,Beg AA,Yu XZ

12-5958 was used in Flow cytometry/Cell sorting to investigate the role of c-Rel in transplant tolerance and graft-versus-host disease (GVHD), showing that it is an essential transcription factor for the development of acute GVHD.

Sun Sep 01 00:00:00 EDT 2013

Thymic damage, impaired negative selection, and development of chronic graft-versus-host disease caused by donor CD4+ and CD8+ T cells.

Journal of immunology (Baltimore, Md. : 1950)

Wu T,Young JS,Johnston H,Ni X,Deng R,Racine J,Wang M,Wang A,Todorov I,Wang J,Zeng D

12-5958 was used in Flow cytometry/Cell sorting to evaluate the contribution of T cells to the induction of chronic graft-versus-host disease.

Mon Jul 01 00:00:00 EDT 2013

The MSHA strain of Pseudomonas aeruginosa activated TLR pathway and enhanced HIV-1 DNA vaccine immunoreactivity.

PloS one

Hou J,Liu Y,Liu Y,Shao Y

12-5958 was used in Flow cytometry/Cell sorting to demonstrate the utility of mannose-sensitive hemagglutination pilus Pseudomonas aeruginosa as an adjuvant to a DNA vaccine.

Wed Apr 03 00:00:00 EDT 2013

Prevention of GVHD while sparing GVL effect by targeting Th1 and Th17 transcription factor T-bet and RORγt in mice.

Blood

Yu Y,Wang D,Liu C,Kaosaard K,Semple K,Anasetti C,Yu XZ

12-5958 was used in Flow cytometry/Cell sorting to indicate that GVHD can be prevented by targeting Th1 and Th17 transcription factors without offsetting GVL activity.

Thu Nov 03 00:00:00 EDT 2011

Stress-regulated targeting of the NKG2D ligand Mult1 by a membrane-associated RING-CH family E3 ligase.

Journal of immunology (Baltimore, Md. : 1950)

Nice TJ,Deng W,Coscoy L,Raulet DH

12-5958 was used in Flow cytometry/Cell sorting to investigate the regulation of cell surface expression of the NKG2D ligand, Mult1.

Mon Nov 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: class I major histocompatibility antigen H-2Kd; H-2 class I histocompatibility antigen, K-B alpha chain; H-2 class I histocompatibility antigen, K-D alpha chain; H-2 class I histocompatibility antigen, K-K alpha chain; H-2 class I histocompatibility antigen, K-Q alpha chain; H-2 class I histocompatibility antigen, K-W28 alpha chain; H-2K(B); H-2K(K); H-2K(Q); MHC class I antigen; MHC class I H2-K-b-alpha-2 cell surface glycoprotein; MHC class I heavy chain H2-K; MHC H2-K transplantation antigen

Gene Aliases: H-2K; H-2K(d); H2-K; H2-K1; K-f

UniProt ID: (Mouse) P01901

Entrez Gene ID: (Mouse) 14972

Molecular Function: major histocompatibility complex protein

Function(s)

positive regulation of T cell mediated cytotoxicity immune system process antigen processing and presentation of peptide antigen via MHC class I antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent immune response antigen processing and presentation antigen processing and presentation of exogenous peptide antigen via MHC class I defense response to bacterium inner ear development

Process(es)

receptor binding protein binding beta-2-microglobulin binding peptide binding peptide antigen binding T cell receptor binding poly(A) RNA binding TAP binding protein heterodimerization activity

MHC Class I (H-2Kb) Monoclonal Antibody (AF6-88.5.5.3), PE, eBioscience™

Product Details

12-5958-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

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