Staining of C57BL/6 (left) and BALB/c (right) splenocytes with 0.06 µg of Mouse IgG2a K Isotype Control APC (Product # 17-4724) (blue histogram) or 0.06 µg of Anti-Mouse MHC Class I (H-2Kb) APC (purple histogram). Total viable cells were used for analysis.
|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG2a, kappa|
|Storage buffer||PBS, pH 7.2, with 0.1% gelatin|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.125 µg/test|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 1 publications below|
Description: This AF6-22.214.171.124 monoclonal antibody reacts with the H-2Kb MHC class I alloantigen. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8. Reactivity to other haplotypes (e.g. d, f, j, k, p, q, r, s, u, and v) has not been observed.
Applications Reported: This AF6-126.96.36.199 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This AF6-188.8.131.52 antibody has been tested by flow cytometric analysis on C57Bl/6 splenocytes. This antibody can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||Inhibition of proliferation of MIs- and Ia-reactive cloned T cells by a monoclonal antibody against a determinant shared by I-A and I-E.||Wall KA,Lorber MI,Loken MR,McClatchey S,Fitch FW||Journal of immunology (Baltimore, Md. : 1950) (131:1056)||1983|