Invitrogen

MHC Class I (H-2Kb) Monoclonal Antibody (AF6-88.5.5.3), APC, eBioscience™

Product Image Gallery

MHC Class I (H-2Kb) Antibody in Flow Cytometry (Flow) — Staining of C57BL/6 (left) and BALB/c (right) splenocytes with 0.06 µg of Mouse IgG2a K Isotype Control APC (Product # 17-4724-81) (blue histogram) or 0.06 µg of Anti-Mouse MHC Class I (H-2Kb) APC (purple histogram). Total viable cells were used for analysis.

Product Details

17-5958-82

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	0.125 µg/test	View 31 publications 31 publications

Product Specifications
Species Reactivity	Mouse
Published species	Human, Mouse
Host/Isotype	Mouse / IgG2a, kappa
Recommended Isotype Control	Mouse IgG2a kappa Isotype Control (eBM2a), APC, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	AF6-88.5.5.3
Conjugate	APC Alexa Fluor 700 APC-eFluor 780 Biotin eFluor 450 FITC NovaFluor Blue 510 NovaFluor Blue 610-70S NovaFluor Blue 660-120S NovaFluor Red 700 PE PE-Cyanine7 PE-eFluor 610 PerCP-eFluor 710 Super Bright 436 Super Bright 600 Super Bright 702
Excitation/Emission Max	651/660 nm View spectra
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_1311280

Product Specific Information

Description: This AF6-88.5.5.3 monoclonal antibody reacts with the H-2Kb MHC class I alloantigen. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8. Reactivity to other haplotypes (e.g. d, f, j, k, p, q, r, s, u, and v) has not been observed.

Applications Reported: This AF6-88.5.5.3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This AF6-88.5.5.3 antibody has been tested by flow cytometric analysis on C57Bl/6 splenocytes. This antibody can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

A mouse model for the study of anti-tumor T cell responses in Kras-driven lung adenocarcinoma.

Cell reports methods

Fitzgerald B,Connolly KA,Cui C,Fagerberg E,Mariuzza DL,Hornick NI,Foster GG,William I,Cheung JF,Joshi NS

17-5958-82 was used in Flow Cytometry to report the development of the immunogenic KP × NINJA (inversion inducible joined neoantigen) (KP-NINJA) LUAD model.

Mon Sep 27 00:00:00 EDT 2021

Mesenchymal stem cell exosome-derived miR-223 alleviates acute graft-versus-host disease via reducing the migration of donor T cells.

Stem cell research & therapy

Liu W,Zhou N,Liu Y,Zhang W,Li X,Wang Y,Zheng R,Zhang Y

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 17-5958-82) in Flow Cytometry

Fri Feb 26 00:00:00 EST 2021

Frequent amplification of HDAC genes and efficacy of HDAC inhibitor chidamide and PD-1 blockade combination in soft tissue sarcoma.

Journal for immunotherapy of cancer

Que Y,Zhang XL,Liu ZX,Zhao JJ,Pan QZ,Wen XZ,Xiao W,Xu BS,Hong DC,Guo TH,Shen LJ,Fan WJ,Chen HY,Weng DS,Xu HR,Zhou PH,Zhang YZ,Niu XH,Zhang X

17-5958-82 was used in Flow Cytometry to conclude that the combination of chidamide and anti-programmed cell death 1 therapy represents a potentially important strategy for soft tissue sarcoma.

Mon Feb 01 00:00:00 EST 2021

An Integrated Epigenomic and Transcriptomic Map of Mouse and Human αβ T Cell Development.

Immunity

Chopp LB,Gopalan V,Ciucci T,Ruchinskas A,Rae Z,Lagarde M,Gao Y,Li C,Bosticardo M,Pala F,Livak F,Kelly MC,Hannenhalli S,Bosselut R

17-5958-82 was used in Flow Cytometry to identify mouse and human αβ thymocyte developmental trajectories.

Tue Dec 15 00:00:00 EST 2020

An Integrated Epigenomic and Transcriptomic Map of Mouse and Human αβ T Cell Development.

Immunity

Chopp LB,Gopalan V,Ciucci T,Ruchinskas A,Rae Z,Lagarde M,Gao Y,Li C,Bosticardo M,Pala F,Livak F,Kelly MC,Hannenhalli S,Bosselut R

17-5958-82 was used in Flow Cytometry to identify mouse and human αβ thymocyte developmental trajectories.

Tue Dec 15 00:00:00 EST 2020

Intravenous injection of the oncolytic virus M1 awakens antitumor T cells and overcomes resistance to checkpoint blockade.

Cell death & disease

Liu Y,Cai J,Liu W,Lin Y,Guo L,Liu X,Qin Z,Xu C,Zhang Y,Su X,Deng K,Yan G,Liang J

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 17-5958-82) in Flow Cytometry

Sat Dec 12 00:00:00 EST 2020

IFN-λ Enhances Constitutive Expression of MHC Class I Molecules on Thymic Epithelial Cells.

Journal of immunology (Baltimore, Md. : 1950)

Benhammadi M,Mathé J,Dumont-Lagacé M,Kobayashi KS,Gaboury L,Brochu S,Perreault C

17-5958-82 was used in Flow Cytometry to show unanticipated variation in MHC I expression by ECs from various sites and provides compelling evidence that superior expression of MHC I in TECs is crucial for proper thymocyte education.

Tue Sep 01 00:00:00 EDT 2020

Combining DNMT and HDAC6 inhibitors increases anti-tumor immune signaling and decreases tumor burden in ovarian cancer.

Scientific reports

Moufarrij S,Srivastava A,Gomez S,Hadley M,Palmer E,Austin PT,Chisholm S,Diab N,Roche K,Yu A,Li J,Zhu W,Lopez-Acevedo M,Villagra A,Chiappinelli KB

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 17-5958-82) in Flow Cytometry

Wed Feb 26 00:00:00 EST 2020

Platelet-derived β2m regulates age related monocyte/macrophage functions.

Aging

Hilt ZT,Ture SK,Mohan A,Arne A,Morrell CN

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 17-5958-82) in Flow Cytometry

Wed Dec 18 00:00:00 EST 2019

Trans-omics Impact of Thymoproteasome in Cortical Thymic Epithelial Cells.

Cell reports

Ohigashi I,Tanaka Y,Kondo K,Fujimori S,Kondo H,Palin AC,Hoffmann V,Kozai M,Matsushita Y,Uda S,Motosugi R,Hamazaki J,Kubota H,Murata S,Tanaka K,Katagiri T,Kosako H,Takahama Y

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 17-5958-82) in Flow Cytometry

Tue Nov 26 00:00:00 EST 2019

Localized Treatment with Oncolytic Adenovirus Delta-24-RGDOX Induces Systemic Immunity against Disseminated Subcutaneous and Intracranial Melanomas.

Clinical cancer research : an official journal of the American Association for Cancer Research

Jiang H,Shin DH,Nguyen TT,Fueyo J,Fan X,Henry V,Carrillo CC,Yi Y,Alonso MM,Collier TL,Yuan Y,Lang FF,Gomez-Manzano C

17-5958 was used in Flow cytometry/Cell sorting to show that localized intratumoral injection of Delta-24-RGDOX induces an in situ antovaccination of the treated melanoma, the effect of which changes the immune landscape of the treated mice, resulting in systemic immunity against disseminated s.c. and i.c. tumors.

Fri Nov 15 00:00:00 EST 2019

CXCL14 suppresses human papillomavirus-associated head and neck cancer through antigen-specific CD8⁺ T-cell responses by upregulating MHC-I expression.

Oncogene

Westrich JA,Vermeer DW,Silva A,Bonney S,Berger JN,Cicchini L,Greer RO,Song JI,Raben D,Slansky JE,Lee JH,Spanos WC,Pyeon D

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 17-5958-82) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2019

Tumor-intrinsic response to IFNγ shapes the tumor microenvironment and anti-PD-1 response in NSCLC.

Life science alliance

Bullock BL,Kimball AK,Poczobutt JM,Neuwelt AJ,Li HY,Johnson AM,Kwak JW,Kleczko EK,Kaspar RE,Wagner EK,Hopp K,Schenk EL,Weiser-Evans MC,Clambey ET,Nemenoff RA

17-5958-82 was used in Flow Cytometry to demonstrate that targeted enhancement of tumor-intrinsic IFNγ signaling can induce a cascade of changes associated with increased therapeutic vulnerability.

Sat Jun 01 00:00:00 EDT 2019

Downregulation of Blimp1 inhibits the maturation of bone marrow-derived dendritic cells.

International journal of molecular medicine

Xiao J,Zhang J,Li X,Dai X,Wang J,He Y,Wei L,Shi J,Gong N

17-5958 was used in Flow cytometry/Cell sorting to demonstrate that transduction with lenti-shRNA-Blimp1 (lentiviral-mediated B-lymphocyte-induced maturation protein-1 shRNA) modulated the maturation of DCs via MHC-II molecule suppression and inhibited alloreactive T cell activation.

Fri Feb 01 00:00:00 EST 2019

Quiescent Tissue Stem Cells Evade Immune Surveillance.

Immunity

Agudo J,Park ES,Rose SA,Alibo E,Sweeney R,Dhainaut M,Kobayashi KS,Sachidanandam R,Baccarini A,Merad M,Brown BD

17-5958 was used in Flow cytometry/Cell sorting to identify a link between stem cell quiescence, antigen presentation, and immune evasion.

Tue Feb 20 00:00:00 EST 2018

Contractile actomyosin arcs promote the activation of primary mouse T cells in a ligand-dependent manner.

PloS one

Hong J,Murugesan S,Betzig E,Hammer JA

17-5958 was used in Flow cytometry/Cell sorting to investigate the ligand-dependent contribution made by contractile actomyosin arcs populating the peripheral supramolecular activation cluster region of the immunological synapse to T cell receptor microcluster transport and proximal signaling in primary mouse T cells.

Mon Oct 16 00:00:00 EDT 2017

Sec61 blockade by mycolactone inhibits antigen cross-presentation independently of endosome-to-cytosol export.

Proceedings of the National Academy of Sciences of the United States of America

Grotzke JE,Kozik P,Morel JD,Impens F,Pietrosemoli N,Cresswell P,Amigorena S,Demangel C

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 17-5958-82) in Flow Cytometry

Tue Jul 18 00:00:00 EDT 2017

Cross-Presentation of Soluble and Cell-Associated Antigen by Murine Hepatocytes Is Enhanced by Collectrin Expression.

Journal of immunology (Baltimore, Md. : 1950)

Dolina JS,Cechova S,Rudy CK,Sung SJ,Tang WW,Lee J,Hahn YS,Le TH

17-5958 was used in Flow cytometry/Cell sorting to identify the functional role of collectrin in the liver.

Wed Mar 15 00:00:00 EDT 2017

G-CSF-Induced Suppressor IL-10+ Neutrophils Promote Regulatory T Cells That Inhibit Graft-Versus-Host Disease in a Long-Lasting and Specific Way.

Journal of immunology (Baltimore, Md. : 1950)

Perobelli SM,Mercadante AC,Galvani RG,Gonçalves-Silva T,Alves AP,Pereira-Neves A,Benchimol M,Nóbrega A,Bonomo A

17-5958 was used in Flow cytometry/Cell sorting to study the role of incoming IL-10+ neutrophils from G-CSF-treated donor spleen in inhibiting acute graft-versus-host disease.

Tue Nov 01 00:00:00 EDT 2016

HDAC Inhibitors Enhance T-Cell Chemokine Expression and Augment Response to PD-1 Immunotherapy in Lung Adenocarcinoma.

Clinical cancer research : an official journal of the American Association for Cancer Research

Zheng H,Zhao W,Yan C,Watson CC,Massengill M,Xie M,Massengill C,Noyes DR,Martinez GV,Afzal R,Chen Z,Ren X,Antonia SJ,Haura EB,Ruffell B,Beg AA

17-5958 was used in Flow cytometry/Cell sorting to test whether strategies that increase T-cell infiltration to tumours can be efficacious in enhancing immunotherapy response.

Mon Aug 15 00:00:00 EDT 2016

Deletion of Wiskott-Aldrich syndrome protein triggers Rac2 activity and increased cross-presentation by dendritic cells.

Nature communications

Baptista MA,Keszei M,Oliveira M,Sunahara KK,Andersson J,Dahlberg CI,Worth AJ,Liedén A,Kuo IC,Wallin RP,Snapper SB,Eidsmo L,Scheynius A,Karlsson MC,Bouma G,Burns SO,Forsell MN,Thrasher AJ,Nylén S,Westerberg LS

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 17-5958-82) in Flow Cytometry

Mon Jul 18 00:00:00 EDT 2016

Metabolic reprogramming of alloantigen-activated T cells after hematopoietic cell transplantation.

The Journal of clinical investigation

Nguyen HD,Chatterjee S,Haarberg KM,Wu Y,Bastian D,Heinrichs J,Fu J,Daenthanasanmak A,Schutt S,Shrestha S,Liu C,Wang H,Chi H,Mehrotra S,Yu XZ

17-5958 was used in Flow cytometry/Cell sorting to study the metabolic reprogramming of donor T cells following allogeneic haematopoietic cell transplantation.

Fri Apr 01 00:00:00 EDT 2016

Identities of P2 and P3 Residues of H-2Kb-Bound Peptides Determine Mouse Ly49C Recognition.

PloS one

Marquez EA,Kane KP

17-5958 was used in Flow cytometry/Cell sorting to identify two adjacent amino acid residues, P2 and P3, in the peptide binding groove of H-2kb, which determine mouse Ly49C specificity.

Wed Mar 30 00:00:00 EDT 2016

Anti-angiogenic efficacy of 5'-triphosphate siRNA combining VEGF silencing and RIG-I activation in NSCLCs.

Oncotarget

Yuan D,Xia M,Meng G,Xu C,Song Y,Wei J

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 17-5958-82) in Flow Cytometry

Tue Oct 06 00:00:00 EDT 2015

Epitope Prediction Assays Combined with Validation Assays Strongly Narrows down Putative Cytotoxic T Lymphocyte Epitopes.

Vaccines

Ip PP,Nijman HW,Daemen T

17-5958 was used in Flow cytometry/Cell sorting to investigate the most promising C57BL/6 mouse-restricted Hepatitis C virus (HCV) MHC class I epitopes and validate these epitopes in vitro and in vivo.

Tue Mar 24 00:00:00 EDT 2015

IL-3 and CSF-1 interact to promote generation of CD11c+ IL-10-producing macrophages.

PloS one

Sheng KC,Herrero LJ,Taylor A,Hapel AJ,Mahalingam S

17-5958 was used in Flow cytometry/Cell sorting to investigate the mechanisms of regulation of hematopoiesis.

Tue Dec 30 00:00:00 EST 2014

The N-terminal domain of NLRC5 confers transcriptional activity for MHC class I and II gene expression.

Journal of immunology (Baltimore, Md. : 1950)

Neerincx A,Jakobshagen K,Utermöhlen O,Büning H,Steimle V,Kufer TA

17-5958 was used in Flow cytometry/Cell sorting to demonstrate the function of NLRC5 and CIITA in MHC gene regulation.

Mon Sep 15 00:00:00 EDT 2014

Alphavirus-based vaccines encoding nonstructural proteins of hepatitis C virus induce robust and protective T-cell responses.

Molecular therapy : the journal of the American Society of Gene Therapy

Ip PP,Boerma A,Regts J,Meijerhof T,Wilschut J,Nijman HW,Daemen T

17-5958 was used in Flow cytometry/Cell sorting to study an rSFV-based vector which induces robust T-cell activity and has a potential for the treatment of HCV infections.

Tue Apr 01 00:00:00 EDT 2014

Protection of islet grafts through transforming growth factor-β-induced tolerogenic dendritic cells.

Diabetes

Thomas DC,Wong FS,Zaccone P,Green EA,Wållberg M

17-5958 was used in Flow cytometry/Cell sorting to investigate methods to achieve long-term β-islet graft tolerance for diabetes type 1, showing that islets can be protected through expression of TGF-β.

Sun Sep 01 00:00:00 EDT 2013

Multifaceted therapeutic targeting of ovarian peritoneal carcinomatosis through virus-induced immunomodulation.

Molecular therapy : the journal of the American Society of Gene Therapy

Gujar S,Dielschneider R,Clements D,Helson E,Shmulevitz M,Marcato P,Pan D,Pan LZ,Ahn DG,Alawadhi A,Lee PW

17-5958 was used in Flow cytometry/Cell sorting to develop a virus-based therapy that suppresses immunomodulation and targets ovarian peritoneal carcinomatosis.

Fri Feb 01 00:00:00 EST 2013

Novel murine dendritic cell lines: a powerful auxiliary tool for dendritic cell research.

Frontiers in immunology

Fuertes Marraco SA,Grosjean F,Duval A,Rosa M,Lavanchy C,Ashok D,Haller S,Otten LA,Steiner QG,Descombes P,Luber CA,Meissner F,Mann M,Szeles L,Reith W,Acha-Orbea H

17-5958 was used in Flow cytometry/Cell sorting to report the generation of new murine DC lines, named MutuDC, originating from cultures of splenic CD8α conventional DC (cDC) tumours.

Tue Nov 20 00:00:00 EST 2012

Dynamic in situ cytometry uncovers T cell receptor signaling during immunological synapses and kinapses in vivo.

Immunity

Moreau HD,Lemaître F,Terriac E,Azar G,Piel M,Lennon-Dumenil AM,Bousso P

17-5958 was used in Flow cytometry/Cell sorting to examine how T cells sense TCR ligands of varying affinities in vivo.

Fri Aug 24 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: class I major histocompatibility antigen H-2Kd; H-2 class I histocompatibility antigen, K-B alpha chain; H-2 class I histocompatibility antigen, K-D alpha chain; H-2 class I histocompatibility antigen, K-K alpha chain; H-2 class I histocompatibility antigen, K-Q alpha chain; H-2 class I histocompatibility antigen, K-W28 alpha chain; H-2K(B); H-2K(K); H-2K(Q); MHC class I antigen; MHC class I H2-K-b-alpha-2 cell surface glycoprotein; MHC class I heavy chain H2-K; MHC H2-K transplantation antigen

Gene Aliases: H-2K; H-2K(d); H2-K; H2-K1; K-f

UniProt ID: (Mouse) P01901

Entrez Gene ID: (Mouse) 14972

Molecular Function: major histocompatibility complex protein

Function(s)

positive regulation of T cell mediated cytotoxicity immune system process antigen processing and presentation of peptide antigen via MHC class I antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent immune response antigen processing and presentation antigen processing and presentation of exogenous peptide antigen via MHC class I defense response to bacterium inner ear development

Process(es)

receptor binding protein binding beta-2-microglobulin binding peptide binding peptide antigen binding T cell receptor binding poly(A) RNA binding TAP binding protein heterodimerization activity

MHC Class I (H-2Kb) Monoclonal Antibody (AF6-88.5.5.3), APC, eBioscience™

Product Details

17-5958-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

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