Invitrogen

MHC Class II (I-A) Monoclonal Antibody (NIMR-4), PE, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

6 Published Figures

19 References

View all (2) MHC Class II (I-A) antibodies

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Protocols

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MHC Class II (I-A) Antibody in Flow Cytometry (Flow) — Fig. 9 Physical interaction between TMPs and the DC surface is critical for TMP-mediated DC activation. a Schematic diagram of the transwell system (left). After co-incubation for 2 h, DCs were acid washed and evaluated for TCRbeta and V5G transfer (upper panel), followed continued incubation for 24 h. The expression of MHC class II, CD80, CD86, and CD40 on DCs (CD11c + ) was analysed by flow cytometry and expressed as histograms and mean fluorescence intensity (MFI). Data represent the mean of three experiments +- SEM. * P < 0.01 vs. c . b 4-TMPs from anti-CD3-coated surfaces were treated with DCs as depicted in the schematic diagram (left) and incubated as described in a . TCRbeta, CD86, and CD40 presentation on DCs (CD11c + ) was analysed as described in a . Data represent the mean of three experiments +- SEM. * P < 0.01 vs. c . c Transfer of TCRs to DCs in vivo. OTII TCR transgenic mice were administered OVA257-264 and OVA323-339 (100 mug; i.p.) in PBS, respectively. After 48 h, draining lymph nodes were taken and dissociated, and cells were treated by acid buffer and stained for the indicated surface markers. Boxed areas represent TCRbeta expression on DCs. Data are represented by one of three independent experiments with similar results. * P < 0.01 vs. none or OVA257-264

MHC Class II (I-A) Antibody — Figure-3 TLR-activation decreases the ubiquitination of MHC-II in BCG infected MPhis & siRNA knock-down of MARCH1 enhance surface expression of MHC-II levels a ) BMA.A4 MPhis of duplicate wells were activated with TLR-ligands (1 mug/mL for 18 hr), infected with BCG (MOI=1) for 4 hr, lysed and pooled. An antibody to MHC-II antibody was used to capture MHC-II from lysates at 4degC overnight and the immunoprecipitate was subjected to western blot using an antibody to polyubiquitin. Panel shows that MPhi-activation with TLR-agonists for 9/7/5/3 and 1/2 decrease the levels of ubiquitinated MHC-II relative to BCG infected control (one of three similar experiments shown). b ) MPhis were treated with siRNA vs. MARCH1 (Santa Cruz Biotechnology) or scrambled control siRNA for 18 hr, rested for 18 hr, and infected with BCG for 4 hr. Surface expression of MHC-II was measured using flow cytometry, and data were expressed as log positive cells. Knock-down of MARCH1 increases surface expression of MHC-II, even in BCG infected macrophages. Data averaged for two separate experiments (p value indicated for groups compared, t test). {TM}

Product Details

12-5322-81

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	0.25 µg/test	View 19 publications 19 publications

Product Specifications
Species Reactivity	Mouse
Published species	Mouse
Host/Isotype	Rat / IgG2b
Recommended Isotype Control	Rat IgG2b kappa Isotype Control (eB149/10H5), PE, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	NIMR-4
Conjugate	PE FITC
Excitation/Emission Max	565/576 nm View spectra
Form	Liquid
Concentration	0.1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_465930

Product Specific Information

Description: The monoclonal antibody NIMR-4 reacts specifically with a non-polymorphic I-A-encoded epitope on MHC Class II antigens. Class II antigens are predominantly expressed on antigen-presenting cells including B lymphocytes, macrophages, dendritic cells and certain epithelial cells. MHC Class II molecules are important for the selection of the TCR repertoire during the processes of positive and negative selection. MHC Class II expression is required for the positive selection of CD4+ T cells.

Applications Reported: This NIMR-4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This NIMR-4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

MHC class II molecules are heterodimers of non-covalently associated alpha (31-34 kDa) and beta (26-29 kDa) chains. Major histocompatibility complex class II antigen presentation requires the participation of lysosomal proteases in two convergent processes. First, the antigens endocytosed by the antigen presenting cells must be broken down into antigenic peptides. Second, class II molecules are synthesized with their peptide-binding site blocked by invariant chain (Ii), and they aquire the capacity to bind antigens only after Ii has been degraded in the compartments where peptides reside. MHC class II molecules present exogenously derived antigen to CD4+ T lymphocytes, which are usually T helper cells. CD4 interacts with non-polymorphic residues of MHC class II.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

A macrophage-T cell coculture model for severe tissue injury-induced T cell death.

STAR protocols

Zhu J,Cao J,Liesz A,Roth S

12-5322-81 was used in Flow Cytometry to present an in vitro model for cell-cell interactions between innate (macrophages) and adaptive (T cells) immune cells.

Fri Dec 17 00:00:00 EST 2021

Chronic T cell proliferation in brains after stroke could interfere with the efficacy of immunotherapies.

The Journal of experimental medicine

Heindl S,Ricci A,Carofiglio O,Zhou Q,Arzberger T,Lenart N,Franzmeier N,Hortobagyi T,Nelson PT,Stowe AM,Denes A,Edbauer D,Liesz A

12-5322-81 was used in Flow Cytometry to show a persistent accumulation of T cells in mice and human autopsy samples for more than 1 mo after stroke.

Mon Aug 02 00:00:00 EDT 2021

Senolytics prevent mt-DNA-induced inflammation and promote the survival of aged organs following transplantation.

Nature communications

Iske J,Seyda M,Heinbokel T,Maenosono R,Minami K,Nian Y,Quante M,Falk CS,Azuma H,Martin F,Passos JF,Niemann CU,Tchkonia T,Kirkland JL,Elkhal A,Tullius SG

12-5322 was used in Flow cytometry/Cell sorting to show that cell-free mitochondrial DNA (cf-mt-DNA) released by senescent cells accumulates with aging and augments immunogenicity.

Thu Aug 27 00:00:00 EDT 2020

Non-redundant Functions of IL-6 Produced by Macrophages and Dendritic Cells in Allergic Airway Inflammation.

Frontiers in immunology

Gubernatorova EO,Gorshkova EA,Namakanova OA,Zvartsev RV,Hidalgo J,Drutskaya MS,Tumanov AV,Nedospasov SA

12-5322 was used in Flow cytometry/Cell sorting to indicate that IL-6 plays a pathogenic role in the HDM-induced asthma model and that lung macrophages and dendritic cells are the predominant sources of pathogenic IL-6 but contribute differently to the disease.

Wed Oct 02 00:00:00 EDT 2019

T cell microvilli constitute immunological synaptosomes that carry messages to antigen-presenting cells.

Nature communications

Kim HR,Mun Y,Lee KS,Park YJ,Park JS,Park JH,Jeon BN,Kim CH,Jun Y,Hyun YM,Kim M,Lee SM,Park CS,Im SH,Jun CD

Published figure using MHC Class II (I-A) monoclonal antibody (Product # 12-5322-81) in Flow Cytometry

Fri Sep 07 00:00:00 EDT 2018

PDL-1 Blockade Prevents T Cell Exhaustion, Inhibits Autophagy, and Promotes Clearance of Leishmania donovani.

Infection and immunity

Habib S,El Andaloussi A,Elmasry K,Handoussa A,Azab M,Elsawey A,Al-Hendy A,Ismail N

Published figure using MHC Class II (I-A) monoclonal antibody (Product # 12-5322-81) in Flow Cytometry

Fri Jun 01 00:00:00 EDT 2018

Human-induced pluripotent stem cell-derived macrophages and their immunological function in response to tuberculosis infection.

Stem cell research & therapy

Hong D,Ding J,Li O,He Q,Ke M,Zhu M,Liu L,Ou WB,He Y,Wu Y

12-5322 was used in Flow cytometry/Cell sorting to characterise human induced pluripotent stem cell-derived macrophages and their response to Bacillus Calmette-Guerin infection.

Mon Feb 26 00:00:00 EST 2018

RANKL-mediated harmonious dialogue between fetus and mother guarantees smooth gestation by inducing decidual M2 macrophage polarization.

Cell death & disease

Meng YH,Zhou WJ,Jin LP,Liu LB,Chang KK,Mei J,Li H,Wang J,Li DJ,Li MQ

12-5322 was used in Flow cytometry/Cell sorting to investigate the effect of RANKL from human embryonic trophoblasts and maternal decidual stromal cells on decidual macrophage differentiation and maternal-fetal immune tolerance.

Thu Oct 12 00:00:00 EDT 2017

Myeloid-derived NF-κB negative regulation of PU.1 and c/EBP-β-driven pro-inflammatory cytokine production restrains LPS-induced shock.

Innate immunity

Vanoni S,Tsai YT,Waddell A,Waggoner L,Klarquist J,Divanovic S,Hoebe K,Steinbrecher KA,Hogan SP

12-5322 was used in Flow cytometry/Cell sorting to study the increased mortality in mice lacking p65 in the myeloid lineage compared with wild type mice upon ultra-pure lipopolysaccharide challenge.

Wed Feb 01 00:00:00 EST 2017

Activation of PPARγ by a Natural Flavonoid Modulator, Apigenin Ameliorates Obesity-Related Inflammation Via Regulation of Macrophage Polarization.

EBioMedicine

Feng X,Weng D,Zhou F,Owen YD,Qin H,Zhao J,Huang Y,Chen J,Fu H,Yang N,Chen D,Li J,Tan R,Shen P

12-5322 was used in Flow cytometry/Cell sorting to demonstrate that PPARγ is bound and activated by apigenin with multiple knock-on effects culminating in M2 macrophage polarisation.

Fri Jul 01 00:00:00 EDT 2016

Dendritic cell dysfunction and diabetic sensory neuropathy in the cornea.

The Journal of clinical investigation

Gao N,Yan C,Lee P,Sun H,Yu FS

12-5322 was used in Flow cytometry/Cell sorting to show that dendritic cells mediate sensory nerve innervation and regeneration through ciliary neurotrophic factor while diabetes reduces dendritic cell populations.

Mon May 02 00:00:00 EDT 2016

CD80 and CD86 knockdown in dendritic cells regulates Th1/Th2 cytokine production in asthmatic mice.

Experimental and therapeutic medicine

Li JG,DU YM,Yan ZD,Yan J,Zhuansun YX,Chen R,Zhang W,Feng SL,Ran PX

12-5322 was used in Flow cytometry/Cell sorting to show how CD80 and CD86 knockdown in dendritic cells regulates Th1/Th2 cytokine production in asthmatic mice.

Tue Mar 01 00:00:00 EST 2016

Dietary lactosucrose suppresses influenza A (H1N1) virus infection in mice.

Bioscience of microbiota, food and health

Kishino E,Takemura N,Masaki H,Ito T,Nakazawa M

12-5322 was used in Flow cytometry/Cell sorting to examine the effects of lactosucrose (4(G)-β-D-galactosylsucrose) on influenza A virus infections in mice.

Mon Nov 23 00:00:00 EST 2015

Injectable cryogel-based whole-cell cancer vaccines.

Nature communications

Bencherif SA,Warren Sands R,Ali OA,Li WA,Lewin SA,Braschler TM,Shih TY,Verbeke CS,Bhatta D,Dranoff G,Mooney DJ

12-5322 was used in Flow cytometry/Cell sorting to investigate cancer vaccines based on injectable whole-cell cryogels.

Wed Aug 12 00:00:00 EDT 2015

Mouse bone marrow-derived mesenchymal stem cells inhibit leukemia/lymphoma cell proliferation in vitro and in a mouse model of allogeneic bone marrow transplant.

International journal of molecular medicine

Song N,Gao L,Qiu H,Huang C,Cheng H,Zhou H,Lv S,Chen L,Wang J

12-5322 was used in Flow cytometry/Cell sorting to determine that MSCs may be safe and effective when used in allogeneic BMT for the treatment of haemotological malignancies.

Wed Jul 01 00:00:00 EDT 2015

Lung dendritic cells undergo maturation and polarization towards a T helper type 2-stimulating phenotype in a mouse model of asthma: Role of nerve growth factor.

Experimental and therapeutic medicine

Qin Q,Wang Z,Pan P,Cao Z,Xia Q,Tan H,Hu C

Published figure using MHC Class II (I-A) monoclonal antibody (Product # 12-5322-81) in Flow Cytometry

Sat Nov 01 00:00:00 EDT 2014

BCG vaccine mediated reduction in the MHC-II expression of macrophages and dendritic cells is reversed by activation of Toll-like receptors 7 and 9.

Cellular immunology

Bakhru P,Sirisaengtaksin N,Soudani E,Mukherjee S,Khan A,Jagannath C

Published figure using MHC Class II (I-A) monoclonal antibody (Product # 12-5322-81) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2014

A type I IFN-Flt3 ligand axis augments plasmacytoid dendritic cell development from common lymphoid progenitors.

The Journal of experimental medicine

Chen YL,Chen TT,Pai LM,Wesoly J,Bluyssen HA,Lee CK

12-5322 was used in Flow cytometry/Cell sorting to define a critical role for the FL/IFN-I/Flt3 axis in plasmacytoid dendritic cell differentiation from common lymphoid progenitors.

Mon Nov 18 00:00:00 EST 2013

Aryl hydrocarbon receptor (AhR) regulates silica-induced inflammation but not fibrosis.

Toxicological sciences : an official journal of the Society of Toxicology

Beamer CA,Seaver BP,Shepherd DM

12-5322 was used in Flow cytometry/Cell sorting to investigate the numerous physiological roles of the aryl hydrocarbon receptor using a mouse model.

Sun Apr 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: H-2 class II histocompatibility antigen, A beta chain; histocompatibility 2, class II antigen A, beta 1; major histocompatibility complex class II beta chain; MHC class II antigen A beta; MHC class II H2-IA-beta-psi; response to metastatic cancers 1

Gene Aliases: Abeta; AI845868; H-2Ab; H2-Ab; H2-Ab1; H2-iabeta; I-Abeta; Ia-2; Ia2; IAb; Rmcs1

UniProt ID: (Mouse) P14483

Entrez Gene ID: (Mouse) 14961

Molecular Function: major histocompatibility complex protein

Function(s)

B cell affinity maturation immune system process immunoglobulin production involved in immunoglobulin mediated immune response humoral immune response mediated by circulating immunoglobulin antigen processing and presentation of peptide or polysaccharide antigen via MHC class II positive regulation of antigen processing and presentation positive regulation of T-helper 1 type immune response immune response antigen processing and presentation antigen processing and presentation of exogenous peptide antigen via MHC class II negative regulation of T cell proliferation positive regulation of alpha-beta T cell activation antigen processing and presentation of peptide antigen cellular response to interferon-gamma

Process(es)

protein binding transcription factor binding toxic substance binding ubiquitin protein ligase binding peptide antigen binding protein heterodimerization activity repressing transcription factor binding

MHC Class II (I-A) Monoclonal Antibody (NIMR-4), PE, eBioscience™

Product Details

12-5322-81

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

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Product Specific Information

Target Information

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