|Flow Cytometry (Flow)||0.25 µg/test|
|Flow Cytometry (Flow)||See 1 publications below|
|Tested Species reactivity||Mouse, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Mouse / IgG2a, kappa|
|Recommended Isotype Control||Mouse IgG2a kappa Isotype Control, FITC, eBioscience™|
|Excitation/Emission Profile||View spectra|
|Storage buffer||PBS, pH 7.2, with 0.1% gelatin|
|Contains||0.09% sodium azide|
|Storage conditions||4° C, store in dark, DO NOT FREEZE!|
Description: The 14-4-4S monoclonal antibody reacts with the mouse and rat major histocompatibility complex class II, I-E subregion-encoded glycoprotein in mice strains of H-2^k, H-2^d, H-2^p and H-2^r. H-2^b, H-2^f and H-2^s haplotypes do not express the I-E antigen. MHC class II is present on B cells, monocytes, macrophages, and dendritic cells. The 14-4-4S mAb has been reported to block antigen presentation in in vitro assays. This antibody cross-reacts with rat.
Applications Reported: 14-4-4S has been reported for use in flow cytometric analysis.
Applications Tested: The 14-4-4S antibody has been tested by flow cytometric analysis of mouse splenocytes and can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: AI323765; E-alpha-f; H-2 class II histocompatibility antigen, E-D alpha chain; H-2 class II histocompatibility antigen, E-K alpha chain; H-2 class II histocompatibility antigen, E-U alpha chain; H-2Ea; H2-IE-alpha; histocompatibility 2, class II antigen E alpha; I-E alpha MHC class II; I-Ealpha; Ia-3; Ia3; MGC117888; MHC class II antigen E alpha; MHC class II protein
Gene Aliases: AI323765; E-alpha-f; H-2Ea; H2-Ea; H2-Ea-ps; I-Ealpha; Ia-3; Ia3
UniProt ID: (Mouse) P14439
Entrez Gene ID: (Mouse) 100504404
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