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Staining of rat splenocytes with Mouse IgG1 kappa Isotype Control FITC (top, open histogram) or Mouse IgG1 kappa Isotype Control PE (bottom, open histogram) and Anti-Rat MHC Class II FITC (top, filled histogram) or Anti-Rat MHC Class II PE (bottom, filled histogram, A16208). Total viable cells were used for analysis.
|Tested species reactivity||Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||rat MHC class II protein|
|Storage buffer||proprietary buffer|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MHC class II molecules are heterodimers of non-covalently associated alpha (31-34 kDa) and beta (26-29 kDa) chains. Major histocompatibility complex class II antigen presentation requires the participation of lysosomal proteases in two convergent processes. First, the antigens endocytosed by the antigen presenting cells must be broken down into antigenic peptides. Second, class II molecules are synthesized with their peptide-binding site blocked by invariant chain (Ii), and they aquire the capacity to bind antigens only after Ii has been degraded in the compartments where peptides reside. MHC class II molecules present exogenously derived antigen to CD4+ T lymphocytes, which are usually T helper cells. CD4 interacts with non-polymorphic residues of MHC class II.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.