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|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Goat / IgG|
|Immunogen||Synthetic peptide sequence (QESPYVSGMKTCH) corresponding to the C-terminus amino acids of MID2|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||5-10 µg/ml|
|Western Blot (WB)||0.1-0.3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is expected to recognize both human isoforms of this protein.
It is predicted to react with bovine, canine and porcine based on sequence homology.
The protein encoded by this gene is a member of the tripartite motif family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
FLJ37715; FLJ41813; FXY2; MID2; midin 2; midin-2; midline 2; midline defect 2; midline-2; probable E3 ubiquitin-protein ligase MID2; RING finger protein 60; RNF60; RP6-191P20.2; TRIM1; tripartite motif protein 1; tripartite motif-containing protein 1
FXY2; MID2; MRX101; RNF60; TRIM1