|Immunocytochemistry (ICC)||20 ug/ml|
|Immunofluorescence (IF)||20 ug/ml|
|Immunohistochemistry (IHC)||5 µg/ml|
|Western Blot (WB)||1-2 ug/ml|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 15 amino acid peptide near the amino terminus of human MINA.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
A suggested positive control is human heart tissue lysate.
PA5-20909 can be used with blocking peptide PEP-1023.
MINA is nuclear localized, myc-inducible protein that is thought to play a role in mammalian cell proliferation. Treatment of cancer cells lines such as the colon cancer cell line SW680 with siRNA against MINA inhibits cell growth, demonstrating that MINA may be a potential therapeutic target. MINA regulates several genes related to cell adhesion and metabolism that have also been shown to be regulated by c-Myc, but also regulates other genes whose expression are not modulated by c-Myc such as EGFR, IL-6 and HGF. MINA has also been found to act as a repressor to IL-4 expression in T cells, indicating that it may also play a role in T cell differentiation and genetic variation in T helper type 2 bias.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 60S ribosomal protein L27a histidine hydroxylase; Bifunctional lysine-specific demethylase and histidyl-hydroxylase MINA; Histone lysine demethylase MINA; MDIG, MINA53, NO52; MINA; mineral dust induced gene protein; Mineral dust-induced gene protein; MYC-induced nuclear antigen; myc-induced nuclear antigen, 53 kDa; Nucleolar protein 52; Ribosomal oxygenase 2; Ribosomal oxygenase MINA; ROX
Gene Aliases: 1810047J07Rik; 2410057H13Rik; 3830408E23Rik; AI449204; MDIG; MINA; MINA53; NO52; RIOX2; ROX
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