|Tested species reactivity||Human, Non-human primate|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant fragment of MLH1 (aa381-483) expressed in E. Coli.|
|Contains||0.03% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||1/200 - 1/1000|
|Immunohistochemistry (IHC)||1/200 - 1/1000|
|Western Blot (WB)||1/500 - 1/2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-15431 targets MLH1 in IF, IHC, and WB applications and shows reactivity with Human and Non-human primate samples.
The MA5-15431 immunogen is purified recombinant fragment of MLH1 (aa381-483) expressed in E. Coli.
MA5-15431 detects MLH1 which has a predicted molecular weight of approximately 85kDa.
This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer found in HNPCC. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described, but their full-length natures have not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.