|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant full length protein (Human).|
|Storage buffer||0.05M sodium phosphate with 0.2% gelatin|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||9-11 µg/ml|
|Western Blot (WB)||1-1.5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (Paraffin) (IHC (P))||See 8 publications below|
MA1-90218 detects MLH1 from human samples.
MA1-90218 has been successfully used in immunohistochemistry (paraffin tissue), Western blot applications.
The MA1-90218 immunogen is: Recombinant full length protein (Human).
This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described, but their full-length natures have not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Clinical and histomolecular endometrial tumor characterization of patients at-risk for Lynch syndrome in South of Brazil.
MA1-90218 was used in immunohistochemistry - paraffin section to measure the frequency of mismatch repair deficiencies in women diagnosed with endometrial cancer who are at-risk for Lynch syndrome
|Cossio SL,Koehler-Santos P,Pessini SA,Mónego H,Edelweiss MI,Meurer L,Errami A,Coffa J,Bock H,Saraiva-Pereira ML,Ashton-Prolla P,Prolla JC||Familial cancer (9:131)||2010|
Clinical implications of microsatellite instability and MLH1 gene inactivation in sporadic insulinomas.
MA1-90218 was used in immunohistochemistry - paraffin section to discuss biomarkers for insulinomas
|Mei M,Deng D,Liu TH,Sang XT,Lu X,Xiang HD,Zhou J,Wu H,Yang Y,Chen J,Lu CM,Chen YJ||The Journal of clinical endocrinology and metabolism (94:3448)||2009|
Expression of hMLH1 and hMSH2 proteins in pleomorphic adenoma of minor salivary glands: relationship with clinical and histologic findings.
MA1-90218 was used in immunohistochemistry - paraffin section to assess the relationship between clinicopathological features and expression of hMLH1 and hMSH2 proteins in pleomorphic adenoma of minor salivary glands
|Tobón-Arroyave SI,Flórez-Moreno GA,Jaramillo-Cárdenas JF,Arango-Uribe JD,Isaza-Guzmán DM,Rendón-Henao J||Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics (108:227)||2009|
Impact of microsatellite instability (MSI) on survival in high grade endometrial carcinoma.
MA1-90218 was used in immunohistochemistry - paraffin section to correlate microstellite instability and other clinicopathologic markers with survival of patients with high grade endometrial carcinoma
|Arabi H,Guan H,Kumar S,Cote M,Bandyopadhyay S,Bryant C,Shah J,Abdul-Karim FW,Munkarah AR,Ali-Fehmi R||Gynecologic oncology (113:153)||2009|
|Not Applicable||Not Cited||
Oncoprotein Bcl-2 and microsatellite instability are associated with disease-free survival and treatment response in colorectal cancer.
MA1-90218 was used in immunohistochemistry - paraffin section to determine the expression of Bcl-2, hMLH1, and hMSH2 in patients with advanced colorectal cancer and correlate their expression with therapeutic response and disease outcome
|Bendardaf R,Lamlum H,Ristamäki R,Syrjänen K,Pyrhönen S||Oncology reports (20:999)||2008|
Thymidylate synthase and microsatellite instability in colorectal cancer: implications for disease free survival, treatment response and survival with metastases.
MA1-90218 was used in immunohistochemistry - paraffin section to evaluate thymidylate synthase and microsatellite instability in colorectal cancer
|Bendardaf R,Lamlum H,Ristamäki R,Korkeila E,Syrjänen K,Pyrhönen S||Acta oncologica (Stockholm, Sweden) (47:1046)||2008|
Sebaceous carcinoma of the eyelids: thirty cases from Japan.
MA1-90218 was used in immunohistochemistry - paraffin section to characterize thirty cases of sebaceous carcinoma of the eyelids
|Izumi M,Mukai K,Nagai T,Matsubayashi J,Iwaya K,Chiu CS,Goto H||Pathology international (58:483)||2008|
Overexpression of hMSH2 and hMLH1 protein in certain gastric cancers and their surrounding mucosae.
MA1-90218 was used in immunohistochemistry - paraffin section to quantify expression levels of mismatch repair proteins hMSH2, hMLH1, PCNA and Ki67 in the mucosae collected from the patients with gastric cancer
|Li M,Liu L,Wang Z,Wang L,Liu Z,Xu G,Lu S||Oncology reports (19:401)||2008|