|Immunohistochemistry (Paraffin) (IHC (P))||1:200|
|Immunohistochemistry (IHC)||See 4 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from near C-terminus of human MMP-1 protein|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is placenta, bladder, breast or ovarian carcinoma.
Matrix metalloproteinases (MMP) are proteolytic enzymes capable of degrading connective tissue components. Imbalanced secretion of certain MMP or disturbances in the differential control of MMP by tissue inhibitor of MMPs (TIMPs) is implicated in the invasive potential of malignant tumors. Interstitial collagenase (MMP-1), 72kDa gelatinase A / type IV collagenase (MMP-2), and 92kDa gelatinase B / type IV collagenase (MMP-9) dissolve extracellular matrix (ECM) and may initiate and promote angiogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CLG; CLGN; Fibroblast collagenase; Interstitial collagenase; matrix metallopeptidase 1 (interstitial collagenase); matrix metalloprotease 1; matrix metalloproteinase 1; Matrix metalloproteinase-1; MMP-1
Gene Aliases: CLG; CLGN; MMP1
UniProt ID: (Human) P03956
Entrez Gene ID: (Human) 4312
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