|Tested species reactivity||Human|
|Published species reactivity||Rat, Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Human recombinant MMP-10 (stromelysin-2 protein)|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 4 publications below|
MA5-14233 targets MMP-10 (Stromelysin-2) in IP and WB applications and shows reactivity with Human samples.
The MA5-14233 immunogen is human recombinant MMP-10 (stromelysin-2 protein).
Matrix metalloproteinases (MMP) are proteolytic enzymes capable of degrading extracellular matrix. Stromelysin, a member of the matrix metalloproteinase family, demonstrates wide substrate specificity with the ability to degrade proteoglycan, fibronectin, laminin, casein, and the nonhelical region of collagen. The two forms of stromelysin (SL), types 1 (MMP-3) and 2 (MMP-10), share 82% sequence homology, but exhibit differences in cellular synthesis and inducibility by cytokines and growth factors in vitro.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Induction of pulmonary fibrosis by cerium oxide nanoparticles.
MA5-14233 was used in immunohistochemistry to study the ability of cerium oxide nanoparticles to induce pulmonary fibrosis in a rat model
|Ma JY,Mercer RR,Barger M,Schwegler-Berry D,Scabilloni J,Ma JK,Castranova V||Toxicology and applied pharmacology (262:255)||2012|
Matrix metalloproteinase induction in fibrosis and fibrotic nodule formation due to silica inhalation.
MA5-14233 was used in immunohistochemistry to study fibrosis and fibrotic nodule formation following silica inhalation and the role of MMPs
|Scabilloni JF,Wang L,Antonini JM,Roberts JR,Castranova V,Mercer RR||American journal of physiology. Lung cellular and molecular physiology (288:L709)||2005|
Expression and localisation of matrix metalloproteinases and their natural inhibitors in fistulae of patients with Crohn's disease.
MA5-14233 was used in immunohistochemistry to investigate the expression and the role of matrix metalloproteinases in intestinal fistulae
|Kirkegaard T,Hansen A,Bruun E,Brynskov J||Gut (53:701)||2004|
Extracellular matrix and matrix metalloproteinase changes in human corneas after complicated laser-assisted in situ keratomileusis (LASIK).
MA5-14233 was used in immunohistochemistry to study changes in corneal extracellular matrix and MMPs following complicated laser-assisted in situ keratomileusis
|Maguen E,Zorapapel NC,Zieske JD,Ninomiya Y,Sado Y,Kenney MC,Ljubimov AV||Cornea (21:95)||2002|