|Immunocytochemistry (ICC)||1 ug/ml|
|Immunofluorescence (IF)||1 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:200|
|Western Blot (WB)||1-3µg/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein corresponding to amino acids 221–471 of human MMP-13|
|Contains||0.09% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
This antibody is predicted to react with mouse, rat, non-human primate and rabbit based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Matrix metallopeptidase 13 belongs to the matrix metalloproteinase peptidase M10A subfamily. The inactive polypeptide undergoes serine protease cleavage, resulting in an active form containing catalytic and hemopexin-like domains. MMP-13 is involved in the breakdown of the extracellular matrix during tissue remodeling, embryonic development, and reproduction. MMP-13 cleaves type II collagen more efficiently than types I and III.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CLG3; Collagenase 3; MANDP1; matrix metalloproteinase 13; matrix metalloproteinase 13 (collagenase 3); Matrix metalloproteinase-13; MMP-13
Gene Aliases: CLG3; MANDP1; MMP-13; MMP13
UniProt ID: (Human) P45452
Entrez Gene ID: (Human) 4322
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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