Immunofluorescent analysis of MMP-13 in HeLa cells using a MMP-13 recombinant rabbit monoclonal antibody (Product # 701287) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showing nuclear localization of MMP-13.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein corresponding to amino acids 221–471 of human MMP-13|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||2-4µg/10^6 cells|
|Immunocytochemistry (ICC)||1 ug/ml|
|Immunofluorescence (IF)||1 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:200|
|Western Blot (WB)||1-3µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with mouse, rat, non-human primate and rabbit based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Matrix metallopeptidase 13 belongs to the matrix metalloproteinase peptidase M10A subfamily. The inactive polypeptide undergoes serine protease cleavage, resulting in an active form containing catalytic and hemopexin-like domains. MMP-13 is involved in the breakdown of the extracellular matrix during tissue remodeling, embryonic development, and reproduction. MMP-13 cleaves type II collagen more efficiently than types I and III.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.