Immunohistochemistry analysis of MMP-2 showing staining in the cytoplasm of paraffin-embedded human breast carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a MMP-2 (72kDa Collagenase IV) Mouse Monoclonal Antibody (MA5-13590) diluted in 3% BSA-PBS at a dilution of 1:100 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Dog, Rat, Human, Mouse, Chicken|
|Host / Isotype||Mouse / IgG1|
|Clone||CA-4001 or CA719E3C|
|Immunogen||N-terminal peptide APSPIIKFPGD-VAPKTDK of procollagenase IV|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Inhibition Assays (IA)||2-4 µg/ml|
|Western Blot (WB)||0.5-1.0 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13590 targets MMP-2 (72kDa Collagenase IV) in IA and WB applications and shows reactivity with Human, mouse, and Rat samples.
The MA5-13590 immunogen is n-terminal peptide APSPIIKFPGD-VAPKTDK of procollagenase IV.
MMPs are proteolytic enzymes capable of degrading connective tissue components. They have a common mode of activation, a conserved amino acid sequence in the putative metal binding-active site region, and are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs). MMPs and TIMPs play a significant role in regulating angiogenesis. MMP-2; also known as 72kDa collagenase IV or gelatinase A is synthesized as a 631 amino acid proenzyme which is activated by cleavage of the first 80 amino acids into an active form.of 62kDa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Myofibroblasts from salivary gland adenoid cystic carcinomas promote cancer invasion by expressing MMP2 and CXCL12.
MA5-13590 was used in immunohistochemistry to determine the role of myofibroblasts in salivary gland adenoid cystic carcinoma invasiveness
|Guan H,Tan J,Zhang F,Gao L,Bai L,Qi D,Dong H,Zhu L,Li X,Liu T||Histopathology (66:781)||2015|
Matrix metalloproteinase, vitamin A and methylprednisolone effects on experimentally induced amyloid arthropathy.
MA5-13590 was used in immunohistochemistry to study the effects of vitamin A and methylprednisolone on the expression of MMP-1, -2 and -9 in a chick model of amyloid arthropathy
|Sevimli A,Ya¿c¿ A,Bülbül A||Biotechnic and histochemistry : official publication of the Biological Stain Commission (88:336)||2013|
Expression of matrix metalloproteinase-12 is correlated with extracapsular spread of tumor from nodes with metastasis in head and neck squamous cell carcinoma.
MA5-13590 was used in immunohistochemistry to study the relationship between expression of MMP-12 in head and neck squamous cell carcinoma primary tumors and extracapsular spread of lymph node metastases
|Kim JM,Kim HJ,Koo BS,Rha KS,Yoon YH||European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery (270:1137)||2013|
Induction of pulmonary fibrosis by cerium oxide nanoparticles.
MA5-13590 was used in immunohistochemistry to study the ability of cerium oxide nanoparticles to induce pulmonary fibrosis in a rat model
|Ma JY,Mercer RR,Barger M,Schwegler-Berry D,Scabilloni J,Ma JK,Castranova V||Toxicology and applied pharmacology (262:255)||2012|
Gelatinases A and B expression in human colorectal cancer in upper Egypt: a clinicopathological study.
MA5-13590 was used in immunohistochemistry to study the immunohistochemical expression of MMP2 and MMP9 in colorectal cancer in an upper Egypt population
|Omran OM,Thabet M||Ultrastructural pathology (36:108)||2012|
MMP-2 expression in 102 patients with glottic laryngeal cancer.
MA5-13590 was used in immunohistochemistry to study MMP-2 expression in patients with glottic laryngeal cancer and its potential prognostic value
|Mallis A,Teymoortash A,Mastronikolis NS,Werner JA,Papadas TA||European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery (269:639)||2012|
The effects of interferon alpha2b on chemically-induced peritoneal fibrosis and on peritoneal tissue MMP-2 and TIMP-2 levels in rats.
MA5-13590 was used in immunohistochemistry to characterize effects of alpha2b treatment on fibrosis and metalloproteinase levels
|Ucar E,Borazan A,Semerci E,Binici DN,Yaldiz M,Aysal A,Altug E,Kuvandik C,Huzmeli C,Yetim T,Canda S||The Journal of international medical research (38:187)||2010|
Matrix metalloproteinase induction in fibrosis and fibrotic nodule formation due to silica inhalation.
MA5-13590 was used in immunohistochemistry to study fibrosis and fibrotic nodule formation following silica inhalation and the role of MMPs
|Scabilloni JF,Wang L,Antonini JM,Roberts JR,Castranova V,Mercer RR||American journal of physiology. Lung cellular and molecular physiology (288:L709)||2005|
Expression of matrix metalloproteinase (MMP)-2 and MMP-9 in breast cancer with a special reference to activator protein-2, HER2, and prognosis.
MA5-13590 was used in immunohistochemistry to study the prognostic value of MMP2 and MMP9 in breast cancer and their relationship to activator protein-2 and HER2
|Pellikainen JM,Ropponen KM,Kataja VV,Kellokoski JK,Eskelinen MJ,Kosma VM||Clinical cancer research : an official journal of the American Association for Cancer Research (10:7621)||2004|
Expression and localisation of matrix metalloproteinases and their natural inhibitors in fistulae of patients with Crohn's disease.
MA5-13590 was used in immunohistochemistry to investigate the expression and the role of matrix metalloproteinases in intestinal fistulae
|Kirkegaard T,Hansen A,Bruun E,Brynskov J||Gut (53:701)||2004|
Phase-dependent expression of matrix metalloproteinases and their inhibitors in demyelinating canine distemper encephalitis.
MA5-13590 was used in immunohistochemistry to study the phase-dependent expression of MMPs and TIMPs in demyelinating canine distemper encephalitis
|Miao Q,Baumgärtner W,Failing K,Alldinger S||Acta neuropathologica (106:486)||2003|
¿11ß1 integrin-mediated MMP-13-dependent collagen lattice contraction by fibroblasts: evidence for integrin-coordinated collagen proteolysis.
MA5-13590 was used in western blot to study the role of mechanical strain generated by alpha11beta1 integrin in MMP-13-mediated fibroblast collagen lattice remodelling
|Barczyk MM,Lu N,Popova SN,Bolstad AI,Gullberg D||Journal of cellular physiology (228:1108)||2013|
Down-regulation of matrix metalloproteinase-9 by pyrrolidine dithiocarbamate prevented retinal ganglion cell death after transection of optic nerve in rats.
MA5-13590 was used in western blot to study the effects of pyrrolidine dithiocarbamate on MMP2 and MMP9 expresssion and MMP-mediated apoptosis in retinal ganglion cells following transection of the optic nerve
|Sun MH,Chen KJ,Tsao YP,Kao LY,Han WH,Lin KK,Pang JH||Current eye research (36:1053)||2011|
SK-PC-B70M confers anti-oxidant activity and reduces Abeta levels in the brain of Tg2576 mice.
MA5-13590 was used in western blot to study the effect of an oleanolic-glycoside saponin on anti-oxidant activity and Abeta levels in the brain of Tg2576 mice.
|Seo JS,Kim TK,Leem YH,Lee KW,Park SK,Baek IS,Kim KS,Im GJ,Lee SM,Park YH,Han PL||Brain research (1261:100)||2009|
Rosiglitazone Suppresses the Growth and Invasiveness of SGC-7901 Gastric Cancer Cells and Angiogenesis In Vitro via PPARgamma Dependent and Independent Mechanisms.
MA5-13590 was used in western blot to investigate the effect of rosiglitazone on SGC-7901 gastric cancer cells and its mechanism
|He Q,Pang R,Song X,Chen J,Chen H,Chen B,Hu P,Chen M||PPAR research (2008:null)||2008|
The hepatitis B virus X protein promotes hepatocellular carcinoma metastasis by upregulation of matrix metalloproteinases.
MA5-13590 was used in western blot to examine the effect of hepatitis B virus X protein on hepatocellular carcinoma metastasis
|Ou DP,Tao YM,Tang FQ,Yang LY||International journal of cancer (120:1208)||2007|
Derivation and characterization of a Wilms' tumour cell line, WiT 49.
MA5-13590 was used in western blot to derive and characterize a Wilms' tumour cell line
|Alami J,Williams BR,Yeger H||International journal of cancer (107:365)||2003|
GSK-3-specific inhibitor-supplemented hESC medium prevents the epithelial-mesenchymal transition process and the up-regulation of matrix metalloproteinases in hESCs cultured in feeder-free conditions.
MA5-13590 was used in immunocytochemistry to investigate the effect of an inhibitor against GSK-3 on the epithelial-mesenchymal transition process and the maintenance of th pluripotency of stem cells
|Ullmann U,Gilles C,De Rycke M,Van de Velde H,Sermon K,Liebaers I||Molecular human reproduction (14:169)||2008|
Cardiac morphogenesis: matrix metalloproteinase coordination of cellular mechanisms underlying heart tube formation and directionality of looping.
MA5-13590 was used in blocking or activating experiment to study the role of MMPs in cardiac morphogenesis
|Linask KK,Han M,Cai DH,Brauer PR,Maisastry SM||Developmental dynamics : an official publication of the American Association of Anatomists (233:739)||2005|
Matrix metalloproteinases 9 and 2 are necessary for the migration of Langerhans cells and dermal dendritic cells from human and murine skin.
MA5-13590 was used in blocking or activating experiment to study the role of MMP2 and MMP9 in the migration of Langerhans cells and dermal dendritic cells from skin
|Ratzinger G,Stoitzner P,Ebner S,Lutz MB,Layton GT,Rainer C,Senior RM,Shipley JM,Fritsch P,Schuler G,Romani N||Journal of immunology (Baltimore, Md. : 1950) (168:4361)||2002|