|Tested species reactivity||Human|
|Published species reactivity||Rat, Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||APMA (4-aminophenylmercuric acetate) activated human MMP-3|
|Storage buffer||PBS, pH 7.4|
|Tested Applications||Dilution *|
|Affinity Purification (AP)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MS810PABX targets MMP-3 in AP, IF, and WB applications and shows reactivity with Human samples.
The MS810PABX immunogen is aPMA (4-aminophenylmercuric acetate) activated human MMP-3.
MS810PABX detects MMP-3 which has a predicted molecular weight of approximately 43 kDa.
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Immunoexpression of matrix metalloproteinases and their inhibitors in different areas of oral squamous cell carcinoma.
MS810PABX was used in immunohistochemistry to study the expression of MMPs and TIMPs in different histopathological regions of oral squamous cell carcinomas
|Suarez-Roa ML,Asbun-Bojalil J,Ruiz-Godoy LM,Meneses-García AA||Australian dental journal (57:300)||2012|
Creation, validation, and quantitative analysis of protein expression in vascular tissue microarrays.
MS810PABX was used in immunohistochemistry to evaluate the usefulness of tissue microarray technology for protein analysis in vascular segments
|Halushka MK,Cornish TC,Lu J,Selvin S,Selvin E||Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology (19:136)||2010|
Inflammatory infiltrates and neovessels are relevant sources of MMPs in abdominal aortic aneurysm wall.
MS810PABX was used in immunohistochemistry to investigate the expression of matrix metalloproteinases in inflammatory infiltrates and neovessels in abdominal aortic aneurysm wall
|Reeps C,Pelisek J,Seidl S,Schuster T,Zimmermann A,Kuehnl A,Eckstein HH||Pathobiology : journal of immunopathology, molecular and cellular biology (76:243)||2009|
Modulation of extracellular matrix components by metalloproteinases and their tissue inhibitors during degeneration and regeneration of rat sural nerve.
MS810PABX was used in immunohistochemistry and western blot to study the role of MMPs and TIMPs in remodelleing the extracellular matrix during degeneration and regeneration of rat sural nerve
|Gantus MA,Nasciutti LE,Cruz CM,Persechini PM,Martinez AM||Brain research (1122:36)||2006|
Cigarette smoke condensate affects the collagen-degrading ability of human gingival fibroblasts.
MS810PABX was used in western blot to study the effect of cigarette smoke condensate on the collagen-degrading ability of human gingival fibroblasts
|Zhang W,Song F,Windsor LJ||Journal of periodontal research (44:704)||2009|
Nicotine increases the collagen-degrading ability of human gingival fibroblasts.
MS810PABX was used in western blot to study the role of nicotine in increasing the collagen-degrading ability of human gingival fibroblasts
|Zhou J,Olson BL,Windsor LJ||Journal of periodontal research (42:228)||2007|
Interleukin-1 alpha alters the expression of matrix metalloproteinases and collagen degradation by pulp fibroblasts.
MS810PABX was used in western blot to study the effect of IL-1 alpha on the expression of MMPs and collagen degradation by pulp fibroblasts
|Wisithphrom K,Murray PE,Windsor LJ||Journal of endodontics (32:186)||2006|
The in vitro effects of dehydroepiandrosterone on human osteoarthritic chondrocytes.
MS810PABX was used in western blot to study the effects of dehydroepiandrosterone on chondrocytes isolated from human osteoarthritic cartilage
|Jo H,Park JS,Kim EM,Jung MY,Lee SH,Seong SC,Park SC,Kim HJ,Lee MC||Osteoarthritis and cartilage (11:585)||2003|
APMA (4-aminophenylmercuric acetate) activation of stromelysin-1 involves protein interactions in addition to those with cysteine-75 in the propeptide.
MS810PABX was used in ELISA to investigate the mechanism for the activation of stromelysin 1 by APMA
|Galazka G,Windsor LJ,Birkedal-Hansen H,Engler JA||Biochemistry (35:11221)||1996|