|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||APMA (4-Aminophenylmercuric acetate) activated Human stromelysin-1 (SL-1)|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Western Blot (WB)||0.5-1.0 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-14207 targets MMP-3 (Stromelysin-1) in IF and WB applications and shows reactivity with Human samples.
The MA5-14207 immunogen is aPMA (4-Aminophenylmercuric acetate) activated Human stromelysin-1 (SL-1).
Matrix metalloproteinases (MMP) are proteolytic enzymes capable of degrading connective tissue components. Imbalanced secretion of certain MMP or disturbances in the differential control of MMP by tissue inhibitor of MMPs (TIMPs) are implicated in the invasive potential of malignant tumors. Stromelysin (MMP3/Transin) degrades several matrix components including the core proteins of proteoglycans, laminin and non-helical regions of collagens.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Immunoexpression of matrix metalloproteinases and their inhibitors in different areas of oral squamous cell carcinoma.
MA5-14207 was used in immunohistochemistry to study the expression of MMPs and TIMPs in different histopathological regions of oral squamous cell carcinomas
|Suarez-Roa ML,Asbun-Bojalil J,Ruiz-Godoy LM,Meneses-García AA||Australian dental journal (57:300)||2012|
Creation, validation, and quantitative analysis of protein expression in vascular tissue microarrays.
MA5-14207 was used in immunohistochemistry to evaluate the usefulness of tissue microarray technology for protein analysis in vascular segments
|Halushka MK,Cornish TC,Lu J,Selvin S,Selvin E||Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology (19:136)||2010|
Inflammatory infiltrates and neovessels are relevant sources of MMPs in abdominal aortic aneurysm wall.
MA5-14207 was used in immunohistochemistry to investigate the expression of matrix metalloproteinases in inflammatory infiltrates and neovessels in abdominal aortic aneurysm wall
|Reeps C,Pelisek J,Seidl S,Schuster T,Zimmermann A,Kuehnl A,Eckstein HH||Pathobiology : journal of immunopathology, molecular and cellular biology (76:243)||2009|
Cigarette smoke condensate affects the collagen-degrading ability of human gingival fibroblasts.
MA5-14207 was used in western blot to study the effect of cigarette smoke condensate on the collagen-degrading ability of human gingival fibroblasts
|Zhang W,Song F,Windsor LJ||Journal of periodontal research (44:704)||2009|
Nicotine increases the collagen-degrading ability of human gingival fibroblasts.
MA5-14207 was used in western blot to study the role of nicotine in increasing the collagen-degrading ability of human gingival fibroblasts
|Zhou J,Olson BL,Windsor LJ||Journal of periodontal research (42:228)||2007|
Interleukin-1 alpha alters the expression of matrix metalloproteinases and collagen degradation by pulp fibroblasts.
MA5-14207 was used in western blot to study the effect of IL-1 alpha on the expression of MMPs and collagen degradation by pulp fibroblasts
|Wisithphrom K,Murray PE,Windsor LJ||Journal of endodontics (32:186)||2006|
The in vitro effects of dehydroepiandrosterone on human osteoarthritic chondrocytes.
MA5-14207 was used in western blot to study the effects of dehydroepiandrosterone on chondrocytes isolated from human osteoarthritic cartilage
|Jo H,Park JS,Kim EM,Jung MY,Lee SH,Seong SC,Park SC,Kim HJ,Lee MC||Osteoarthritis and cartilage (11:585)||2003|