Immunofluorescent analysis of MMP-9 (92kDa Collagenase IV) (green) showing staining in the cytoplasm of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a MMP-9 (92kDa Collagenase IV) monoclonal antibody (Product # MA5-14220) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Human native 92kDa MMP-9|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Western Blot (WB)||2-4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
MA5-14220 targets MMP-9 (92kDa Collagenase IV) in ICC/IF, IP, and WB applications and shows reactivity with Human samples.
The MA5-14220 immunogen is human native 92kDa MMP-9.
MMPs are a group of enzymes involved in matrix degradation. They share some important characteristics, such as a common mode of activation, a conserved amino acid sequence in the putative metal binding-active site region, and inhibition by specific proteinase inhibitors known as tissue inhibitors of metalloproteinases (TIMPs). Reportedly, MMPs play a crucial role in tumor cell invasion and metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Inflammatory infiltrates and neovessels are relevant sources of MMPs in abdominal aortic aneurysm wall.
MA5-14220 was used in immunohistochemistry to investigate the expression of matrix metalloproteinases in inflammatory infiltrates and neovessels in abdominal aortic aneurysm wall
|Reeps C,Pelisek J,Seidl S,Schuster T,Zimmermann A,Kuehnl A,Eckstein HH||Pathobiology : journal of immunopathology, molecular and cellular biology (76:243)||2009|