Invitrogen

MMP13 Monoclonal Antibody (LIPCO IID1)

MMP13 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

MMP13 Antibody (MA5-14247) in ICC/IF

Immunofluorescence analysis of MMP-13 (Collagenase-3) was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MMP-13 (Collagenase-3) (LIPCO IID1) Mouse Monoclonal Antibody (Product # MA5-14247) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, A... View More

MMP13 Antibody in Immunoprecipitation (IP)

MMP13 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

MMP13 Antibody — Figure 7. Effect of Res treatment on miR-9, NF-kappaB1, IL-6, MMP-13, caspase-3 expression in mouse chondrocytes. The relative (A) miR-9 and (B) NF-kappaB1 mRNA expression levels were determined by RT-qPCR in chondrocytes following treatment with 0, 15 or 30 mM Res for 48 h. (C) The relative NF-kappaB1, IL-6, MMP-13 and caspase-3 protein expression levels were determined by western blot analysis in chondrocytes following treatment with 0, 15 or 30 mM Res for 48 h. Quantification of (D) NF-kappaB1, (E) IL-6, (F) MMP-13 and (G) caspase-3 protein expression. n=3. *P<0.05 vs. control group. miR, microRNA; NF-kappaB1, nuclear factor kappa B subunit 1; IL-6, interleukin-6; MMP-13, matrix metallopeptidase 13; Res, Resveratrol. {TM}

Product Details

MA5-14247

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 19 publications 19 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2 µg/mL

View 2 publications 2 publications

Immunoprecipitation (IP)

2 µg/mL

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Avian, Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

LIPCO IID1

Immunogen

Rat MMP-13 from BC-1 cells

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10985956

Product Specific Information

MA5-14247 targets MMP-13 (Collagenase-3) (Rat) in IF, IP, and WB applications and shows reactivity with mouse and Rat samples.

The MA5-14247 immunogen is rat MMP-13 from BC-1 cells.

Target Information

MMP13 (collagense 3) plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC, and ACAN. MMP13 cleaves tripal helical collagens, including type I, II, and III collagen - but has the highest activity with soluble type II collagen. It can degrade collagen type IV, XIV, and X. MMP13 plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization, and ossification. It is required for normal embryonic bone development and ossificaiton. Mutations affecting the gene can lead to spondyloepimetaphyseal dysplasia Missouri type, metaphyseal anadysplasia 1, and metaphyseal dysplasia Sphar type.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Silencing of long‑chain non‑coding RNA GAS5 in osteoarthritic chondrocytes is mediated by targeting the miR‑34a/Bcl‑2 axis.

Molecular medicine reports

Ji Q,Qiao X,Liu Y,Wang D,Yan J

MA5-14247 was used in Western Blot to indicate that GAS5 participated in the development of OA by regulating the biological behavior of chondrocytes via the miR‚34a/Bcl‚2 axis.

Sun Mar 01 00:00:00 EST 2020

Therapeutic effect of Resveratrol in the treatment of osteoarthritis via the MALAT1/miR-9/NF-κB signaling pathway.

Experimental and therapeutic medicine

Zhang G,Zhang H,You W,Tang X,Li X,Gong Z

Published figure using MMP13 monoclonal antibody (Product # MA5-14247) in Western Blot

Sun Mar 01 00:00:00 EST 2020

The glucocorticoid receptor in monocyte-derived macrophages is critical for cardiac infarct repair and remodeling.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Galuppo P,Vettorazzi S,Hövelmann J,Scholz CJ,Tuckermann JP,Bauersachs J,Fraccarollo D

Published figure using MMP13 monoclonal antibody (Product # MA5-14247) in Immunofluorescence

Wed Nov 01 00:00:00 EDT 2017

The glucocorticoid receptor in monocyte-derived macrophages is critical for cardiac infarct repair and remodeling.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Galuppo P,Vettorazzi S,Hövelmann J,Scholz CJ,Tuckermann JP,Bauersachs J,Fraccarollo D

Published figure using MMP13 monoclonal antibody (Product # MA5-14247) in Immunofluorescence

Wed Nov 01 00:00:00 EDT 2017

Articular cartilage degradation is prevented by tanshinone IIA through inhibiting apoptosis and the expression of inflammatory cytokines.

Molecular medicine reports

Jia PT,Zhang XL,Zuo HN,Lu X,Li L

MA5-14247 was used in Western Blotting to investigate the effect of tanshinone IIA on the degradation of articular cartilage in a rat model of osteoarthritis.

Wed Nov 01 00:00:00 EDT 2017

MicroRNA-27a Inhibits Cell Migration and Invasion of Fibroblast-Like Synoviocytes by Targeting Follistatin-Like Protein 1 in Rheumatoid Arthritis.

Molecules and cells

Shi DL,Shi GR,Xie J,Du XZ,Yang H

Published figure using MMP13 monoclonal antibody (Product # MA5-14247) in Western Blot

Wed Aug 31 00:00:00 EDT 2016

The involvement of oxidants and NF-κB in cytokine-induced MMP-9 synthesis by bone marrow-derived osteoprogenitor cells.

Inflammation research : official journal of the European Histamine Research Society ... [et al.]

Ben-David D,Livne E,Reznick AZ

MA5-14247 was used in western blot to study the role of inflammatory conditions in the induction of MMP-9 production by bone marrow-derived osteoprogenitor cells

Sun Jul 01 00:00:00 EDT 2012

Matrix metalloproteinase-9 in a unique proteoglycan form in avian embryonic growth plate cartilage.

Archives of biochemistry and biophysics

Patchigolla RK,Knudson W,Schmid TM

MA5-14247 was used in western blot to study a novel proteoglycan form of MMP9 isolated from avian embryonic growth plate cartilage

Sun Apr 01 00:00:00 EDT 2012

Expression of matrix metalloproteinase-13 is controlled by IL-13 via PI3K/Akt3 and PKC-δ in normal human dermal fibroblasts.

The Journal of investigative dermatology

Moriya C,Jinnin M,Yamane K,Maruo K,Muchemwa FC,Igata T,Makino T,Fukushima S,Ihn H

MA5-14247 was used in western blot to study the mechanism by which IL-13 regulates MMP-13 expression in normal human dermal fibroblasts

Tue Mar 01 00:00:00 EST 2011

Effects of olive leaf extract and its main component oleuroepin on acute ultraviolet B irradiation-induced skin changes in C57BL/6J mice.

Phytotherapy research : PTR

Sumiyoshi M,Kimura Y

MA5-14247 was used in western blot to investigate the influence of an olive leaf extract and oleuropein on skin inflammation caused by acute UVB irradiation

Thu Jul 01 00:00:00 EDT 2010

mTOR regulates the invasive properties of synovial fibroblasts in rheumatoid arthritis.

Molecular medicine (Cambridge, Mass.)

Laragione T,Gulko PS

MA5-14247 was used in western blot to investigate the role of mTOR in the invasiveness of synovial fibroblasts in rheumatoid arthritis

Wed Dec 22 00:00:00 EST 2010

UV decreases the synthesis of free fatty acids and triglycerides in the epidermis of human skin in vivo, contributing to development of skin photoaging.

Journal of dermatological science

Kim EJ,Jin XJ,Kim YK,Oh IK,Kim JE,Park CH,Chung JH

MA5-14247 was used in western blot to investigate the influence of UV exposure on fatty acid production in the epidermis of human skin

Fri Jan 01 00:00:00 EST 2010

French maritime pine bark (Pinus maritima Lam.) extract (Flavangenol) prevents chronic UVB radiation-induced skin damage and carcinogenesis in melanin-possessing hairless mice.

Photochemistry and photobiology

Kimura Y,Sumiyoshi M

MA5-14247 was used in western blot to investigate the effects of flavangenol on UVB-caused skin damage and skin tumors

Thu Jan 13 00:00:00 EST 2011

Olive leaf extract and its main component oleuropein prevent chronic ultraviolet B radiation-induced skin damage and carcinogenesis in hairless mice.

The Journal of nutrition

Kimura Y,Sumiyoshi M

MA5-14247 was used in western blot to study the protective effects of olive leaf extract and oleuropein on UVB-irradiation induced skin damage

Sun Nov 01 00:00:00 EDT 2009

Differential regulation of MMP-13 expression in two models of experimentally induced periodontal disease in rats.

Archives of oral biology

de Aquino SG,Guimaraes MR,Stach-Machado DR,da Silva JA,Spolidorio LC,Rossa C

MA5-14247 was used in western blot to study MMP-13 expression in two models of experimentally induced periodontal disease in rats

Wed Jul 01 00:00:00 EDT 2009

Ablation of matrix metalloproteinase-9 increases severity of viral myocarditis in mice.

Circulation

Cheung C,Marchant D,Walker EK,Luo Z,Zhang J,Yanagawa B,Rahmani M,Cox J,Overall C,Senior RM,Luo H,McManus BM

MA5-14247 was used in western blot to study the severity of Coxsackievirus B3-induced myocarditis in MMP-8-and MMP-9-deficient mice

Tue Mar 25 00:00:00 EDT 2008

Low concentrations of transforming growth factor-beta-1 induce tubulogenesis in cultured mammary epithelial cells.

BMC developmental biology

Montesano R,Carrozzino F,Soulié P

MA5-14247 was used in western blot to investigate the effect of transforming growth factor-beta-1 at low concentrations on cultured mammary epithelial cells

Thu Feb 08 00:00:00 EST 2007

Inhibition of SMAD2 expression prevents murine palatal fusion.

Developmental dynamics : an official publication of the American Association of Anatomists

Shiomi N,Cui XM,Yamamoto T,Saito T,Shuler CF

MA5-14247 was used in western blot to study the role of SMAD2 in murine palatal fusion

Sat Jul 01 00:00:00 EDT 2006

Halofuginone induces matrix metalloproteinases in rat hepatic stellate cells via activation of p38 and NFkappaB.

The Journal of biological chemistry

Popov Y,Patsenker E,Bauer M,Niedobitek E,Schulze-Krebs A,Schuppan D

MA5-14247 was used in western blot to study the mechanisms underlying the beneficial effects of the semisynthetic plant alkaloid halofuginone in experimental liver fibrosis

Fri Jun 02 00:00:00 EDT 2006

Mechanisms of the inhibitory effect of epigallocatechin-3-gallate on cultured human vascular smooth muscle cell invasion.

Arteriosclerosis, thrombosis, and vascular biology

Cheng XW,Kuzuya M,Nakamura K,Liu Z,Di Q,Hasegawa J,Iwata M,Murohara T,Yokota M,Iguchi A

MA5-14247 was used in western blot to study the mechanisms underlying the effect of epigallocatechin-3-gallate on the invasiveness of cultured human vascular smooth muscle cells

Thu Sep 01 00:00:00 EDT 2005

TGF-beta1 modulates matrix metalloproteinase-13 expression in hepatic stellate cells by complex mechanisms involving p38MAPK, PI3-kinase, AKT, and p70S6k.

American journal of physiology. Gastrointestinal and liver physiology

Lechuga CG,Hernández-Nazara ZH,Domínguez Rosales JA,Morris ER,Rincón AR,Rivas-Estilla AM,Esteban-Gamboa A,Rojkind M

MA5-14247 was used in western blot to study the pathways by which TGF-beta1 regulates MMP-13 expression in hepatic stellate cells

Mon Nov 01 00:00:00 EST 2004

Collagen dissolution by keratinocytes requires cell surface plasminogen activation and matrix metalloproteinase activity.

The Journal of biological chemistry

Netzel-Arnett S,Mitola DJ,Yamada SS,Chrysovergis K,Holmbeck K,Birkedal-Hansen H,Bugge TH

MA5-14247 was used in western blot to study the role of cell surface plasminogen activation and MMP activity in collagen dissolution by keratinocytes

Fri Nov 22 00:00:00 EST 2002

Bioinformatics

Protein Aliases: Collagenase; Collagenase 3; collagenase-1; Collagenase-3; Collagenase3; interstitial collagenase; matrix metallo protease; matrix metalloproteinase 13; Matrix metalloproteinase-13; MMP; MMP-13; MMPs; Procollagenase 3; UMRCASE

View more View less

Gene Aliases: Clg; MMP-13; Mmp1; Mmp13

View more View less

UniProt ID: (Mouse) P33435

View more View less

Entrez Gene ID: (Mouse) 17386, (Rat) 171052

View more View less

Function(s)

fibronectin binding metalloendopeptidase activity calcium ion binding collagen binding peptidase activity metallopeptidase activity zinc ion binding hydrolase activity metal ion binding calcium-dependent protein binding low-density lipoprotein particle receptor binding metalloprotease

Process(es)

endochondral ossification growth plate cartilage development proteolysis extracellular matrix disassembly bone mineralization collagen catabolic process receptor internalization peptide catabolic process cellular protein metabolic process cartilage development bone morphogenesis ossification luteolysis response to hypoxia response to mechanical stimulus embryonic hindlimb morphogenesis response to drug response to estrogen

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

MMP13 Monoclonal Antibody (LIPCO IID1)

MMP13 Antibody (MA5-14247) in ICC/IF

Product Details

MA5-14247

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!