Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Western blot analysis of MMP2 was performed by loading 25 ug of the indicated whole cell lysates and 10ul Prestained Protein Ladder per well onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Fast Blotter (Product # 62288) and blocked with 5% Milk/TBST for at least 1 hour at room temperature. MMP2 was detected at ~74kD using a MMP2 mouse monoclonal antibody (Product # MA1-772) at a concentration of 5ug/ml in blocking buffer overnight at 4°C on a rocking platform, followed by a goat anti-mouse IgG-HRP secondary antibody (Product # 31430) at a dilution of 1:10,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34076).
|Tested species reactivity||Human, Rat|
|Published species reactivity||Bovine|
|Host / Isotype||Mouse / IgG1|
|Clone||F14 P4 D3|
|Immunogen||Synthetic peptide corresponding to residues T(557) S L G L P P D V Q R V D(569) of human MMP-2.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1-2 µg|
|Western Blot (WB)||1:200-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
MA1-772 contains 100 ug of in vitro produced, protein A purified antibody (1 mg/ml) in PBS containing 1 mg/ml BSA and 0.05% sodium azide.
MA1-772 detects MMP-2 from human and rat samples.
MA1-772 has been successfully used in Western blot applications. By Western blot, MA1-772 detects a ~50 kDa band representing MMP-2 from WI-38 cell lysate or rat lung samples.
The MA1-772 immunogen is a synthetic peptide corresponding to residues T(557) S L G L P P D V Q R V D(569) of human MMP-2.
MA1-772 has been successfully used in western blotting and ELISA analysis of MMP2 in human and rat samples.
By Western blot, MA1-772 detects a ~74kDa band representing MMP2 in MCF7 and HT-1080 cell lysate or rat lung.
The matrix metalloproteinases (MMPs) are a family of at least eighteen secreted and membrane-bound zincendopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non-fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins. In general, a signal peptide, a propeptide, and a catalytic domain containing the highly conserved zinc-binding site characterizes the structure of the MMPs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
In vitro assays of rod and cone opsin activity: retinoid analogs as agonists and inverse agonists.
MA1-772 was used in western blot to characterize rod and cone opsin activity using retinoid analogs
|Kono M,Crouch RK||Methods in molecular biology (Clifton, N.J.) (652:85)||2010|
72 kDa gelatinase; 72 kDa type IV collagenase; collagenase type IV-A; Gelatinase A; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase); matrix metalloproteinase 2; Matrix metalloproteinase-2; matrix metalloproteinase-II; MMP-2; neutrophil gelatinase; TBE-1
CLG4; CLG4A; MMP-2; MMP-II; MMP2; MONA; TBE-1