Western blot analysis of MMP2 was performed by loading 25 ug of the indicated whole cell lysates and 10ul Prestained Protein Ladder per well onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Fast Blotter (Product # 62288) and blocked with 5% Milk/TBST for at least 1 hour at room temperature. MMP2 was detected at ~74kD using a MMP2 mouse monoclonal antibody (Product # MA1-772) at a concentration of 5ug/ml in blocking buffer overnight at 4°C on a rocking platform, followed by a goat anti-mouse IgG-HRP secondary antibody (Product # 31430) at a dilution of 1:10,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34076).
|Tested species reactivity||Human, Rat|
|Published species reactivity||Bovine|
|Host / Isotype||Mouse / IgG1|
|Clone||F14 P4 D3|
|Immunogen||Synthetic peptide corresponding to residues T(557) S L G L P P D V Q R V D(569) of human MMP-2.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1-2 µg|
|Western Blot (WB)||1:200-1:500|
|Western Blot (WB)||See 1 publications below|
MA1-772 contains 100 ug of in vitro produced, protein A purified antibody (1 mg/ml) in PBS containing 1 mg/ml BSA and 0.05% sodium azide.
MA1-772 detects MMP-2 from human and rat samples.
MA1-772 has been successfully used in Western blot applications. By Western blot, MA1-772 detects a ~50 kDa band representing MMP-2 from WI-38 cell lysate or rat lung samples.
The MA1-772 immunogen is a synthetic peptide corresponding to residues T(557) S L G L P P D V Q R V D(569) of human MMP-2.
MA1-772 has been successfully used in western blotting and ELISA analysis of MMP2 in human and rat samples.
By Western blot, MA1-772 detects a ~74kDa band representing MMP2 in MCF7 and HT-1080 cell lysate or rat lung.
The matrix metalloproteinases (MMPs) are a family of at least eighteen secreted and membrane-bound zincendopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non-fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins. In general, a signal peptide, a propeptide, and a catalytic domain containing the highly conserved zinc-binding site characterizes the structure of the MMPs.
In vitro assays of rod and cone opsin activity: retinoid analogs as agonists and inverse agonists.
MA1-772 was used in western blot to characterize rod and cone opsin activity using retinoid analogs
|Kono M,Crouch RK||Methods in molecular biology (Clifton, N.J.) (652:85)||2010|
72 kDa gelatinase; 72 kDa type IV collagenase; collagenase type IV-A; gelatinase A; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase); matrix metalloproteinase 2; matrix metalloproteinase-2; matrix metalloproteinase-II; MMP-2; neutrophil gelatinase
CLG4; CLG4A; MMP-2; MMP-II; MMP2; MONA; TBE-1