Samples: Lane 1 - Human Recombinant MMP-2. Lane 2 - Schwann Cell Culture Medium Run on 10% SDS-PAGE, followed by electroblot to nitrocellulose and slot blot immunolabeling. Antibodies: primary - MA1-16640, secondary - anti-mouse-biotin. Detection: avidin-HRP. Chromagen: DAB/H202.
|Tested species reactivity||Guinea pig, Human, Mouse, Rat|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Activated recombinant human MMP-2.|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1 µg/ml|
|Immunohistochemistry (IHC)||5 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||5 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay-Dependent|
|Western Blot (WB)||2-5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is not recommended for Immunohistochemistry on paraffin-embedded sections.
Suggested positive control: lung, nerve, and various tumors.
Loss of negative growth regulation and high invasive potential are neoplastic traits often associated with abnormal expression of matrix matalloproteinases. MMP-2, the signalling mechanism for cells to begin migration, triggers the movement when it splits laminin-5 and exposes and integrin-binding site on this component of epithelial basement membrane. The changed form of laminin was found in tumors and in tissues being altered. The activated form of MMP2, however, is not found in benign tumors. Thus detection of the enzyme is a possible early indicator of tumor activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.