|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Activated recombinant human MMP-2|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.03 µg/ml|
|Immunohistochemistry (IHC)||2 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||2 µg/ml|
|Immunoprecipitation (IP)||1-2 µg/100-500 µg total protein|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
This antibody is specific for both pro and active MMP-2.
Loss of negative growth regulation and high invasive potential are neoplastic traits often associated with abnormal expression of matrix matalloproteinases. MMP-2, the signalling mechanism for cells to begin migration, triggers the movement when it splits laminin-5 and exposes and integrin-binding site on this component of epithelial basement membrane. The changed form of laminin was found in tumors and in tissues being altered. The activated form of MMP2, however, is not found in benign tumors. Thus detection of the enzyme is a possible early indicator of tumor activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Hypoxia Induces Mesenchymal Gene Expression in Renal Tubular Epithelial Cells: An in vitro Model of Kidney Transplant Fibrosis.
MA1-16641 was used in western blot to study the induction of mesenchymal genes in renal tubular epithelial cells subjected to hypoxia and the significance for kidney transplantation-associated fibrosis
|Zell S,Schmitt R,Witting S,Kreipe HH,Hussein K,Becker JU||Nephron extra (3:50)||2013|