Proteintech

MMP2 Monoclonal Antibody (2B10D1)

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MMP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 66366-1-IG (MMP2 antibody) at dilution of 1:300 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

MMP2 Antibody in Western Blot (WB) — Western blot analysis of MMP2 in various cell lines using Proteintech antibody 66366-1-IG ( MMP2 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Product Details

66366-1-IG

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:6,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:150-1:600	-

Product Specifications
Species Reactivity	Human, Mouse, Pig, Rat
Published species	Human
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	2B10D1
Immunogen	MMP2 Fusion Protein Ag25039 (34-469 aa encoded by BC002576) View immunogen
Conjugate	Unconjugated CoraLite488
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: IIKFPGDVA PKTDKELAVQ YLNTFYGCPK ESCNLFVLKD TLKKMQKFFG LPQTGDLDQN TIETMRKPRC GNPDVANYNF FPRKPKWDKN QITYRIIGYT PDLDPETVDD AFARAFQVWS DVTPLRFSRI HDGEADIMIN FGRWEHGDGY PFDGKDGLLA HAFAPGTGVG GDSHFDDDEL WTLGEGQVVR VKYGNADGEY CKFPFLFNGK EYNSCTDTGR SDGFLWCSTT YNFEKDGKYG FCPHEALFTM GGNAEGQPCK FPFRFQGTSY DSCTTEGRTD GYRWCGTTED YDRDKKYGFC PETAMSTVGG NSEGAPCVFP FTFLGNKYES CTSAGRSDGK MWCATTANYD DDRKWGFCPD QGYSLFLVAA HEFGHAMGLE HSQDPGALMA PIYTYTKNFR LSQDDIKGIQ ELYGASPDID LGTGPTPTLG PVTPEIC (34-469 aa encoded by BC002576)

Target Information

MMP (matrix metalloproteinase) are proteolytic enzymes capable of degrading connective tissue components. MMP have a common mode of activation, a conserved amino acid sequence in the putative metal binding-active site region, and are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs). MMPa and TIMPs play a significant role in regulating angiogenesis. MMP2 is synthesized as a 631 amino acid proenzyme which is activated by cleavage of the first 80 amino acids, and contains the basic structure of propeptide, catalytic, and hemopexin domains. The matrix metalloproteinases (MMPs) are a family of at least eighteen secreted and membrane-bound zincendopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non-fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins. In general, a signal peptide, a propeptide, and a catalytic domain containing the highly conserved zinc-binding site characterizes the structure of the MMPs. Functionally, MMP2 is involved in tissue remodeling. Mutations in MMP-2 gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Two transcript variants encoding different isoforms of MMP-2 have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Sinomenine hydrochloride attenuates the proliferation, migration, invasiveness, angiogenesis and epithelial-mesenchymal transition of clear-cell renal cell carcinoma cells via targeting Smad in vitro.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Zhao B,Liu L,Mao J,Liu K,Fan W,Liu J,Zhang Z,Li Q

Fri Dec 01 00:00:00 EST 2017

Bioinformatics

Protein Aliases: 72 kDa gelatinase; 72 kDa type IV collagenase; 72kD gelatinase; 72kD type IV collagenase; 72kDa gelatinase; 72kDa type IV collagenase; CLG 4A; Collagenase; collagenase type IV-A; Gelatinase A; Gelatinase alpha; Mat; matrix metallo protease; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase); matrix metalloproteinase; matrix metalloproteinase 2; Matrix metalloproteinase-2; matrix metalloproteinase-II; MMP; MMP-2; MMPs; neutrophil gelatinase; PEX; Progelatinase A; TBE-1; TBE1

Gene Aliases: CLG4; CLG4A; GelA; MMP-2; MMP-II; MMP2; MONA; TBE-1

UniProt ID: (Human) P08253, (Rat) P33436, (Mouse) P33434

Entrez Gene ID: (Human) 4313, (Pig) 397391, (Rat) 81686, (Mouse) 17390

Molecular Function: metalloprotease