|Tested species reactivity||Human|
|Host / Isotype||Sheep / IgG|
|Immunogen||ProMMP-3 purified from cultured human rheumatoid synovial fibroblasts, shown to be homogeneous by SDS-PAGE.|
|Storage buffer||glycine buffered saline, pH 7.4, with 0.01% benzamidine, 0.1% EACA, 1mM EDTA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-32153 detects MMP3 from human samples.
PA1-32153 has been successfully used in Western blot applications.
The PA1-32153 immunogen is: ProMMP-3 purified from cultured human rheumatoid synovial fibroblasts, shown to be homogeneous by SDS-PAGE.
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.