OriGene

MMP7 Monoclonal Antibody (UMAB168), UltraMAB™

Product Image Gallery

MMP7 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human gastric tumor using anti-MMP7 clone UMAB168 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. UM800061 requires heat-induced epitope retrieval with EDTA pH 8.0 in a presure cooker for 3 minutes at 110°C. The composit image shows two tumors with membranous and cytoplasmic staining in the tumor cells.

MMP7 Antibody in Western Blot (WB) — Western blot of human tissue lysates (15 µg) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen). Dilution: 1:500.

MMP7 Antibody in Peptide array (ARRAY) — OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-MMP7 mouse monoclonal antibody (UM800061). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

Product Details

UM800061CF

Applications	Tested Dilution	Publications
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:200	-
Peptide Array (Array)	Assay-Dependent	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	UMAB168
Immunogen	Human recombinant protein fragment corresponding to amino acids 95-267 of human MMP7 produced in E.coli. View antigen
Conjugate	Unconjugated
Form	lyophilized
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.3, with 8% trehalose
Contains	no preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades proteoglycans, fibronectin, elastin and casein and differs from most MMP family members in that it lacks a conserved C-terminal protein domain. The enzyme is involved in wound healing, and studies in mice suggest that it regulates the activity of defensins in intestinal mucosa. The gene is part of a cluster of MMP genes which localize to chromosome 11q22. 3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: HGNC:7174; Matrilysin; Matrin; matrix metallo protease; matrix metallopeptidase 7 (matrilysin, uterine); matrix metalloproteinase 7 (matrilysin, uterine); Matrix metalloproteinase-7; MMP; MMP 7; MMP-7; MMPs; PUMP 1; Pump 1 protease; Pump-1 protease; uterine matrilysin; Uterine metalloproteinase

Gene Aliases: MMP-7; MMP7; MPSL1; PUMP-1; PUMP1

UniProt ID: (Human) P09237

Entrez Gene ID: (Human) 4316

Molecular Function: metalloprotease