|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Purified recombinant fragment of human MMP9 expressed in E. Coli.|
|Contains||0.03% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1/200 - 1/400|
|Immunofluorescence (IF)||1/200 - 1/1000|
|Immunohistochemistry (IHC)||1/200 - 1/1000|
|Western Blot (WB)||1/500 - 1/2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
MA5-15886 targets MMP9 in FACS, IF, IHC, and WB applications and shows reactivity with Human samples.
The MA5-15886 immunogen is purified recombinant fragment of human MMP9 expressed in E. Coli. .
MA5-15886 detects MMP9 which has a predicted molecular weight of approximately 92kDa.
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
An essential role of syntaxin 3 protein for granule exocytosis and secretion of IL-1¿, IL-1ß, IL-12b, and CCL4 from differentiated HL-60 cells.
MA5-15886 was used in immunocytochemistry to elucidate the intracellular SNARE machinery responsible for the regulation of cytokine secretion and degranulation.
|Naegelen I,Plançon S,Nicot N,Kaoma T,Muller A,Vallar L,Tschirhart EJ,Bréchard S||Journal of leukocyte biology (97:557)||2015|