Invitrogen

MMP9 Monoclonal Antibody (IIA5)

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MMP9 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of MMP-9 showing staining in the cytoplasm of paraffin-embedded human colon carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a MMP-9 monoclonal antibody (Product # MA5-14228) diluted in 3% BSA-PBS at a dilution of 1:200 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

MMP9 Antibody in Western Blot (WB) — Western blot of MMP-9 (92kDa Collagenase IV) using MMP-9 (92kDa Collagenase IV) Monoclonal Antibody (Product # MA5-14228) on HT1080-TPA (Medium) Cells and CCL-4 med Cells.

MMP9 Antibody in Immunocytochemistry (ICC/IF) — Figure 2 Distribution of Ki-67 ( a ) pRb- ( b ), MMP-9- ( c ) and Bax- ( d ) positive cells in the epithelium (Ep), loose ectomesenchyme condensations (lec), loose non-condensing ectomesenchyme (lnc) in formation of the secondary palate (7th-12th week). Data are shown as mean +- SD. Significant differences (Kruskal-Wallis) indicated by * p < 0.05, ** p < 0.001, *** p < 0.0001.

MMP9 Antibody in Immunohistochemistry (IHC) — Fig. 3 Photomicrograph of immunohistochemistry in the ileal sections for MMP-9, NF-kappaB and AQP-4. Anti-MMP-9 immunohistochemistry showing: (A) control group: minimal+ve immunoreaction (arrows) in some cells of the mucosal LP (B) 5-FU group: abundant+ve cytoplasmic immunoreaction (arrows) in the inflammatory cells within the mucosal C.T. and the epithelial cells covering the Vi. (C) Rh-treated group: minimal+ve immunoreaction (arrows) in few inflammatory and epithelial cells. Anti-NF-kappaB immunohistochemistry showing: (D) control group: few+ve cytoplasmic immunoreactions (arrows) in some cells of the mucosal C.T. (E) A 5-FU group: obvious increased+ve immunoreaction (arrows) involving both the cytoplasm and the nuclei of most of the epithelial and inflammatory cells (F) Rh-treated group: some epithelial and inflammatory cells showing+ve immunoreaction (arrows) which is mainly cytoplasmic with few nuclear immunoreactions. Anti-AQP-4 immunohistochemistry showing: (G) control group: obvious+ve AQP-4 immunoreaction (arrows) mostly in the basolateral regions in addition to the cytoplasm of the enterocytes. (H) A 5-FU group: minimal+ve AQP-4 immunoreaction (arrows) in the epithelial cells (I) Rh-treated group: increased+ve AQP-4 immunoreaction (arrows) mainly in the basolateral regions in addition to the cytoplasm of the enterocytes. Scale bars= 20 mum. AQP-4, aquaporin-4; C.T., connective tissue; LP, lamina propria; MMP-9, matrix metalloproteinase-9; NF-kappaB, nuclear factor-

Product Details

MA5-14228

Applications	Tested Dilution	Publications
Western Blot (WB)	1-2 µg/mL	View 8 publications 8 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:500	-
Immunoprecipitation (IP)	Assay-dependent	-
Immunohistochemistry (IHC)	-	View 10 publications 10 publications
Immunocytochemistry (ICC/IF)	-	View 3 publications 3 publications
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Rat
Published species	Dog, Human, Mouse, Rat
Host/Isotype	Mouse / IgG1, kappa
Class	Monoclonal
Type	Antibody
Clone	IIA5
Immunogen	Human native 92kDa MMP-9
Conjugate	Unconjugated
Form	Liquid
Concentration	0.2 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.4, with 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C
RRID	AB_11004645

Product Specific Information

MA5-14228 targets MMP-9 (92kDa Collagenase IV) in IHC, IP, and WB applications and shows reactivity with Human and Rat samples.

The MA5-14228 immunogen is human native 92kDa MMP-9.

Target Information

MMP9 (matrix metallopeptidase 9, GELB, CLG4B) is a matrix metalloproteinase, a family of zinc and calcium-dependent endopeptidases that degrade extracellular matrix proteins. MMP9 is secreted as a 92kDa zymogen and cleavage of pro-MMP9 results in the active enzyme with a molecular weight of 82kDa. MMP9 has a gelatin-binding domain consisting of three fibronectin type II units, a catalytic domain containing the zinc-binding site, a proline-rich type V collagen-homologous domain and a hemopexin-like domain. MMP9 is produced by monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts and endothelial cells, and is involved in inflammatory responses, tissue remodeling, wound healing, tumor growth and metastasis. MMP9 is supplied by bone marrow-derived cells and contributes to skin carcinogenesis. Further, MMP9 degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. Studies have shown that elevated MMP9 is associated with progression of idiopathic atrial fibrillation and aortic aneurysm.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Empagliflozin mitigates type 2 diabetes-associated peripheral neuropathy: a glucose-independent effect through AMPK signaling.

Archives of pharmacal research

Abdelkader NF,Elbaset MA,Moustafa PE,Ibrahim SM

MA5-14228 was used in Immunohistochemistry to explore the potentiality of the selective sodium-glucose cotransporter-2 inhibitor, empagliflozin (EMPA), to ameliorate streptozotocin-induced DPN in rats with insight into its precise signaling mechanism.

Fri Jul 01 00:00:00 EDT 2022

TAGLN and High-mobility Group AT-Hook 2 (HMGA2) Complex Regulates TGF-β-induced Colorectal Cancer Metastasis.

OncoTargets and therapy

Zhou H,Li L,Xie W,Wu L,Lin Y,He X

MA5-14228 was used in Western Blotting to investigate the role of transgelin and high-mobility group AT-hook 2 in the progression of colon cancer.

Sun Apr 17 00:00:00 EDT 2022

TAGLN and High-mobility Group AT-Hook 2 (HMGA2) Complex Regulates TGF-β-induced Colorectal Cancer Metastasis.

OncoTargets and therapy

Zhou H,Li L,Xie W,Wu L,Lin Y,He X

MA5-14228 was used in Western Blotting to investigate the role of transgelin and high-mobility group AT-hook 2 in the progression of colon cancer.

Sun Apr 17 00:00:00 EDT 2022

In Vitro Evaluation of the Anti-Inflammatory Effect of KMUP-1 and In Vivo Analysis of Its Therapeutic Potential in Osteoarthritis.

Biomedicines

Huang SE,Sulistyowati E,Chao YY,Wu BN,Dai ZK,Hsu JH,Yeh JL

MA5-14228 was used in Western Blot to investigate the in vitro anti-inflammatory and in vivo anti-osteoarthritis effects of KMUP-1.

Fri May 28 00:00:00 EDT 2021

In Vitro Evaluation of the Anti-Inflammatory Effect of KMUP-1 and In Vivo Analysis of Its Therapeutic Potential in Osteoarthritis.

Biomedicines

Huang SE,Sulistyowati E,Chao YY,Wu BN,Dai ZK,Hsu JH,Yeh JL

MA5-14228 was used in Western Blot to investigate the in vitro anti-inflammatory and in vivo anti-osteoarthritis effects of KMUP-1.

Fri May 28 00:00:00 EDT 2021

Spatio-Temporal Expression Pattern of Ki-67, pRB, MMP-9 and Bax in Human Secondary Palate Development.

Life (Basel, Switzerland)

Šimić Bilandžija T,Vukojević K,Ćorić A,Vuković Kekez I,Medvedec Mikić I,Lasić Arapović L,Filipović N,Anđelić J,Saraga-Babić M,Kalibović Govorko D

Published figure using MMP9 monoclonal antibody (Product # MA5-14228) in Immunocytochemistry

Sat Feb 20 00:00:00 EST 2021

Disturbed balance in the expression of MMP9 and TIMP3 in cerebral amyloid angiopathy-related intracerebral haemorrhage.

Acta neuropathologica communications

Jäkel L,Kuiperij HB,Gerding LP,Custers EEM,van den Berg E,Jolink WMT,Schreuder FHBM,Küsters B,Klijn CJM,Verbeek MM

MA5-14228 was used in Immunohistochemistry-methylmethacrylate to investigate the expression of MMP9 and TIMP3 in occipital brain tissue of CAA-ICH cases (n = 11) by immunohistochemistry and compared this to the expression in brain tissue of CAA cases without ICH (CAA-non-haemorrhagic, CAA-NH, n = 18).

Mon Jul 06 00:00:00 EDT 2020

Disturbed balance in the expression of MMP9 and TIMP3 in cerebral amyloid angiopathy-related intracerebral haemorrhage.

Acta neuropathologica communications

Jäkel L,Kuiperij HB,Gerding LP,Custers EEM,van den Berg E,Jolink WMT,Schreuder FHBM,Küsters B,Klijn CJM,Verbeek MM

Mon Jul 06 00:00:00 EDT 2020

The beneficial influence of rhubarb on 5-fluorouracil-induced ileal mucositis and the combined role of aquaporin-4, tumour necrosis factor-α, nuclear factor-kappa B & matrix metalloproteinase-9 in rat model: histological study.

Anatomy & cell biology

Kamar SS,Baky MH,Omar AI

Published figure using MMP9 monoclonal antibody (Product # MA5-14228) in Immunohistochemistry

Tue Jun 30 00:00:00 EDT 2020

Astrocyte-derived VEGF increases cerebral microvascular permeability under high salt conditions.

Aging

Deng Z,Zhou L,Wang Y,Liao S,Huang Y,Shan Y,Tan S,Zeng Q,Peng L,Huang H,Lu Z

MA5-14228 was used in Western Blotting to reveal a new axis linking a high salt diet (HSD) to increased cerebral microvascular permeability through a VEGF-initiated inflammatory response.

Mon Jun 22 00:00:00 EDT 2020

A Novel Role of Irbesartan in Gastroprotection against Indomethacin-Induced Gastric Injury in Rats: Targeting DDAH/ADMA and EGFR/ERK Signaling.

Scientific reports

Shahin NN,Abdelkader NF,Safar MM

Published figure using MMP9 monoclonal antibody (Product # MA5-14228) in Immunohistochemistry

Fri Mar 09 00:00:00 EST 2018

The pattern of CD44 and matrix metalloproteinase 9 expression is a useful predictor of ulcerative colitis-associated dysplasia and neoplasia.

Annals of diagnostic pathology

AbdElazeem MA,El-Sayed M

MA5-14228 was used in immunohistochemistry - paraffin section to examine CD44 and MMP-9 expression in different ulcerative colitis lesions

Tue Dec 01 00:00:00 EST 2015

Recruitment of Matrix Metalloproteinase-9 (MMP-9) to the Fibroblast Cell Surface by Lysyl Hydroxylase 3 (LH3) Triggers Transforming Growth Factor-β (TGF-β) Activation and Fibroblast Differentiation.

The Journal of biological chemistry

Dayer C,Stamenkovic I

MA5-14228 was used in immunocytochemistry and western blot to study the recruitment of MMP-9 to cell surface and its effect on fibroblast differentiation

Fri May 29 00:00:00 EDT 2015

In vivo effects of zoledronic acid on oral mucosal epithelial cells.

Oral diseases

Allam E,Allen M,Chu TM,Ghoneima A,Jack Windsor L

MA5-14228 was used in immunohistochemistry to investigate the influence of zoledronic acid on oral mucosal epithelial cells in a dog model

Fri Apr 01 00:00:00 EDT 2011

Noninvasive limb ischemic preconditioning protects against myocardial I/R injury in rats.

The Journal of surgical research

Li SJ,Wu YN,Kang Y,Yin YQ,Gao WZ,Liu YX,Lou JS

MA5-14228 was used in immunohistochemistry to compare the protective effect of noninvasive limb ischemia preconditioning against myocardial ischemia/reperfusion injury in rats

Mon Nov 01 00:00:00 EDT 2010

Human mammary fibroblasts stimulate invasion of breast cancer cells in a three-dimensional culture and increase stroma development in mouse xenografts.

BMC cancer

Olsen CJ,Moreira J,Lukanidin EM,Ambartsumian NS

MA5-14228 was used in western blot to study the effects of fibroblasts on mammary carcinoma cells.

Thu Aug 19 00:00:00 EDT 2010

Cardosins improve neuronal regeneration after cell disruption: a comparative expression study.

Cell biology and toxicology

Duarte AS,Duarte EP,Correia A,Pires E,Barros MT

MA5-14228 was used in western blot to study the use of the plant enzyme cardosin for tissue disaggregation and isolation of rat embryonic neurons

Wed Apr 01 00:00:00 EDT 2009

Impaired leukocyte influx in cervix of postterm women not responding to prostaglandin priming.

Reproductive biology and endocrinology : RB&E

Sahlin L,Stjernholm-Vladic Y,Roos N,Masironi B,Ekman-Ordeberg G

MA5-14228 was used in immunohistochemistry to investigate the mechanism for the non-responsiveness to prostaglandin priming in cervix of postterm women

Tue Sep 02 00:00:00 EDT 2008

Biological and clinical relevance of matrix metalloproteinases 2 and 9 in acute myeloid leukaemias and myelodysplastic syndromes.

European journal of haematology

Travaglino E,Benatti C,Malcovati L,Della Porta MG,Gallì A,Bonetti E,Rosti V,Cazzola M,Invernizzi R

MA5-14228 was used in immunocytochemistry to study the biological and clinical relevance of MMP2 and MMP9 in acute myeloid leukaemias and myelodysplastic syndromes

Sat Mar 01 00:00:00 EST 2008

Pathogenic sequence for dissecting aneurysm formation in a hypomorphic polycystic kidney disease 1 mouse model.

Arteriosclerosis, thrombosis, and vascular biology

Hassane S,Claij N,Lantinga-van Leeuwen IS,Van Munsteren JC,Van Lent N,Hanemaaijer R,Breuning MH,Peters DJ,DeRuiter MC

MA5-14228 was used in immunohistochemistry to study vascular remodeling in a hypomorphic Pkd1(nl/nL) mouse model with dissecting aneurysms in the aorta

Mon Oct 01 00:00:00 EDT 2007

Time course of matrix metalloproteases and tissue inhibitors in bleomycin-induced pulmonary fibrosis.

European journal of histochemistry : EJH

Oggionni T,Morbini P,Inghilleri S,Palladini G,Tozzi R,Vitulo P,Fenoglio C,Perlini S,Pozzi E

MA5-14228 was used in immunohistochemistry to investigate the distribution, expression and activity of matrix metalloproteases and tissue inhibitors during extracellular matrix remodeling in pulmonary fibrosis

Tue Mar 13 00:00:00 EDT 2007

Improved surgical mesh integration into the rat abdominal wall with arginine administration.

Biomaterials

Arbos MA,Ferrando JM,Quiles MT,Vidal J,López-Cano M,Gil J,Manero JM,Peña J,Huguet P,Schwartz-Riera S,Reventós J,Armengol M

MA5-14228 was used in western blot to study the role of arginine administration in improving surgical mesh integration into the rat abdominal wall

Wed Feb 01 00:00:00 EST 2006

Simvastatin suppresses experimental aortic aneurysm expansion.

Journal of vascular surgery

Kalyanasundaram A,Elmore JR,Manazer JR,Golden A,Franklin DP,Galt SW,Zakhary EM,Carey DJ

MA5-14228 was used in western blot to study the therapeutic benefits of simvastatin against experimental aortic aneurysm expansion

Sun Jan 01 00:00:00 EST 2006

Expression and localisation of matrix metalloproteinases and their natural inhibitors in fistulae of patients with Crohn's disease.

Gut

Kirkegaard T,Hansen A,Bruun E,Brynskov J

MA5-14228 was used in immunohistochemistry to investigate the expression and the role of matrix metalloproteinases in intestinal fistulae

Sat May 01 00:00:00 EDT 2004

Expression and localisation of matrix metalloproteinases and their natural inhibitors in fistulae of patients with Crohn's disease.

Gut

Kirkegaard T,Hansen A,Bruun E,Brynskov J

MA5-14228 was used in immunohistochemistry to investigate the expression and the role of matrix metalloproteinases in intestinal fistulae

Sat May 01 00:00:00 EDT 2004

Effect of gamma-synuclein overexpression on matrix metalloproteinases in retinoblastoma Y79 cells.

Archives of biochemistry and biophysics

Surgucheva IG,Sivak JM,Fini ME,Palazzo RE,Surguchov AP

MA5-14228 was used in western blot to study the effect of gamma-synuclein overexpression on MMPs in retinoblastoma cells

Sat Feb 01 00:00:00 EST 2003

Bioinformatics

Protein Aliases: 67 kDa matrix metalloproteinase-9; 82 kDa matrix metalloproteinase-9; 92 kDa gelatinase; 92 kDa type IV collagenase; 92-kDa type IV collagenase; Gelatinase B; Gelatinase-B; GELB; macrophage gelatinase; matrix metallo protease; matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); matrix metalloproteinase 9 (gelatinase B 92-kDa type IV collagenase); matrix metalloproteinase 9 (gelatinase B, 92-kDa type IV collagenase); matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); Matrix metalloproteinase-9; Matrix metalloproteinase9; MMP; MMP-9; MMPs; type V collagenase

Gene Aliases: CLG4B; GELB; MANDP2; MMP-9; MMP9

UniProt ID: (Human) P14780, (Rat) P50282

Entrez Gene ID: (Human) 4318, (Rat) 81687

Molecular Function: metalloprotease

Function(s)

Process(es)

endopeptidase activity metalloendopeptidase activity serine-type endopeptidase activity protein binding collagen binding metallopeptidase activity zinc ion binding identical protein binding fibronectin binding peptidase activity protein complex binding

MMP9 Monoclonal Antibody (IIA5)

Product Details

MA5-14228

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunoprecipitation (IP)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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